N-2-羟乙基哌嗪-N-2-乙磺酸（HEPES），CAS：7365-45-9是一种氢离子缓冲剂，能较长时间控制恒定的pH范围。
分子生物学级HEPES用于T4RNA连接酶进行RNA3'-末端标记，分离与分析核RNA之反应缓冲液组分、预杂交缓冲液缓和杂交缓冲液成分。
溶于水(2.25mol/L,0℃)。熔点210℃。紫外线吸光度A(5%/1cm,250nm,水中)<0.05。
|
Assay(Titration, Dry basis)
|
≥99.0%
|
|
Solubility 1M water
|
Clear, Colorless solution
|
|
pH 1%water solution 25℃
|
4.8~5.6
|
|
Water
|
≤0.5%
|
|
Infrared
|
Complies
|
|
Residue on ignition
|
≤0.1%
|
|
A280,0.1M Water
|
≤0.035
|
|
A260,0.1M Water
|
≤0.045
|
|
Appearance
|
White crystalline powder
|