Cholesterol Oxidase (CHO-PEWL),胆固醇氧化酶(EC 1.1.3)用于测定血清中的总胆固醇。
原理:Cholesterol+2 O2→ 6β-hydroperoxycholest-4-en-3-on+H2O2
SPECIFICATION
Appearance: liquid form (30% sucrose solution)
Activity: 200 U/ml
Storage at -20°C
PROPERTIES
Molecular weight: ca. 59 kDa (gel ?ltration)
Structure: monomer of 60 kDa (SDS-PAGE)
pH Optimum: 6.5–8.0
pH Stability: 3.5–8.5
Optimum temperature: 55°C–65°C
Thermal stability: below 70°C
Stability (liquid form): stable at 25°C for at least one month
Inhibitors Ag2+, Hg2+