果糖基氨基酸氧化酶(FAOX)属于氧化还原酶,是糖化血红蛋白(HbA1c)检测试剂盒(酶法)的主要原料。
原理:Fructosyl-L-amino acid+H2O+O2→ L-Amino acid+Glucosone+H2O2
含有果糖基的氨基酸或肽,在果糖基氨基酸氧化酶的作用下,被特异性地氧化,生成相应的氨基酸、葡萄糖醛酮和过氧化氢。
SPECIFICATION
Appearance yellow lyophilizate
Activity 0.5 U/mg lyophilizate
Stabilizer trehalose
Storage at -20°C
PROPERTIES
Molecular weight ca. 88 kDa (gel ?ltration)
Structure 2 subunits of 44 kDa (SDS-PAGE)
Isoelectric point 4.2
Michaelis constant 5.0104 M (fructosyl-glycine)
pH Optimum 7.5–8.5
pH Stability 6.0–10.0
Optimum temperature 40–50°C
Thermal stability below 45°C
Stability (liquid form) stable at 20°C for at least two weeks
Stability (powder form) stable at 37°C for at least one month
Inhibitors Hg2+, Pb2+