Fructosyl-peptide oxidase(FPOX-CE),果糖基肽氧化酶(EC 1.5.3),用于测定糖尿病患者的糖化血红蛋白(HbA1c)。
原理:Fructosyl-peptide+H2O+O2→ Peptide Glucosone+H2O2
2 H2O2+4-Aminoantipyrine+TOOS→ Quinoneimine dye+4 H2O
SPECIFICATION
Appearance: yellow lyophilizate
Activity: 4 U/mg lyophilizate
Stabilizers: EDTA, glutamate
Storage at -20°C
PROPERTIES
Molecular weight: ca. 60 kDa (gel ?ltration)
Structure: monomer of 52 kDa (SDS-PAGE)
pH Optimum: 7.5–8.0
pH Stability: 6.0–9.5
Optimum temperature: 35–42°C
Thermal stability: below 45°C
Stability (powder form) stable at 37°C for at least one month