β-半乳糖苷酶能够水解通过β-1→4糖苷键连接在单糖、寡糖或糖肽上的半乳糖残基。它主要用于酶联免疫检测中,偶联在抗体上作为标记物。此外,β-半乳糖苷酶还可用于糖类结构分析、乳糖检测和钠离子检测。
Appearance: White powder, lyophilized
Source: E.coli
Enzyme Commission Number: EC 3.2.1.23
CAS Number: 9031-11-2
Storage temperature: -20℃
Specific activity: ≥ 400U/mg protein
Unit definition: One unit will cause the formation of one micromole of ONP from ONPG per min at pH 7.3 at 37℃.
PROPERTIES
Molecular weight: 118 kDa (SDS-PAGE)
Isoelectric point: 4.6
Optimum pH: 7.0
Optimum temperature: 50℃
pH Stability: 5.5~9.5 (25℃,20hr)
Thermal stability: < 45℃ (pH 7.3, 15min)