葡甘露聚糖[魔芋;低黏度] , Glucomannan (konjac; low viscosity) , 货号:P-GLCML
品牌:Megazyme
货号:P-GLCML
中文品名:葡甘露聚糖[魔芋;低黏度]
品名:Glucomannan (konjac; low viscosity)
CASN: 11078-31-2
纯度: >98% (葡萄糖:甘露糖=40:60。 乙酰化。 )
粘度:~ 13 cSt [1% w/v, 30°C 溶于蒸馏水]使用Ostwald C型粘度计
蛋白质:<2%
外观:无味,白色粉末
包装: 4 g
说明:该聚合物包含乙酰基团可增加溶解度。如果加入氢氧化钠(约1% w/v)去除乙酰基团,该化合物在中性pH条件下不能溶解在溶液中。
用途:高纯度葡甘露聚糖[魔芋;低黏度] 用于研究、酶生化分析和体外诊断分析,可作为膳食纤维标准品使用。
参考文献:
Alkaline hydrogen peroxide pretreatment of softwood: Hemicellulose degradation pathways. Alvarez-Vasco, C. & Zhang, X. (2013). Bioresource Technology, 150, 321-327.
A revised architecture of primary cell walls based on biomechanical changes induced by substrate-specific endoglucanases. Park, Y. B. & Cosgrove, D. J. (2012). Plant Physiology, 158(4), 1933-1943.
Methodologies for the extraction and analysis of konjac glucomannan from corms of Amorphophallus konjac K. Koch. Chua, M., Chan, K., Hocking, T. J., Williams, P. A., Perry, C. J. & Baldwin, T. C. (2012). Carbohydrate Polymers, 87(3), 2202-2210.
Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-β-mannosidase from Aspergillus niger BK01. Bien-Cuong, D., Thi-Thu, D., Berrin, J. G., Haltrich, D., Kim-Anh, T., Sigoillot, J. C. & Yamabhai, M. (2009). Microbial Cell Factories, 8(1), 59.
Efficient recombinant expression and secretion of a thermostable GH26 mannan endo-1,4-β-mannosidase from Bacillus licheniformis in Escherichia coli. Songsiriritthigul, C., Buranabanyat, B., Haltrich, D. & Yamabhai, M. (2010). Microbial Cell Factories, 9(1), 20.
Influence of a mannan binding family 32 carbohydrate binding module on the activity of the appended mannanase. Mizutani, K., Fernandes, V. O., Karita, S., Luís, A. S., Sakka, M., Kimura, T., Jackson, A., Zhang, X., Fontes, C. M. G. A., Gilbert, H. J. & Sakka, K. (2012). Applied and Environmental Microbiology, 78(14), 4781-4787.
Structural and Thermodynamic Dissection of Specific Mannan Recognition by a Carbohydrate Binding Module, TmCBM27. Boraston, A. B., Revett, T. J., Boraston, C. M., Nurizzo, D. & Davies, G. J. (2003). Structure, 11(6), 665-675.
Mannan transglycosylase: a novel enzyme activity in cell walls of higher plants. Schröder, R., Wegrzyn, T. F., Bolitho, K. M. & Redgwell, R. J. (2004). Planta, 219(4), 590-600.
Xyloglucans of monocotyledons have diverse structures. Hsieh, Y. S. & Harris, P. J. (2009). Molecular Plant, 2(5), 943-965.
Cellulose microfibril angles and cell-wall polymers in different wood types of Pinus radiata. Brennan, M., McLean, J. P., Altaner, C. M., Ralph, J. & Harris, P. J. (2012). Cellulose, 19(4), 1385-1404.
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