RPMI 1640 Medium

Directions
Dissolve 10.2 g of the medium in distilled water
and adjust the volume to 1,000 mL. Sterilize by
autoclaving at 121°C for 15 minutes. Cool the
sterilized medium to room temperature and add a
proper amount of sterile 10% sodium bicarbonate.
(pH will be 7.1 – 7.4 at 37°C, in an atmosphere of
5% CO2 in air by adding 18 – 30 mL of sodium
bicarbonate solution.) Aseptically add 0.3 g Lglutamine
and the desire amount or serum.
Summary
RPMI 1640 is a medium for suspension culture
investigated by George E. Moore, former director
of Roswell Park Memorial Institute. Up to now,
such media were not autoclavable because of
decomposition or deterioration of the
constituents, so that sterilization had been
performed by filtration using a membrane filter.
RPMI 1640 Medium “Nissui” 2 is an autoclavablepowdered
medium specially manufactured to
overcome the difficulties associated with filtersterilized
media, such as contamination by
Mycoplasma. This medium has growth promoting
effect to primary cultures of mouse and human
leukemia cells, various kinds of cell lines and
KATO-III cells.
Storage and Expiration
Close tightly and store at 2-5°C in a dark and dry
place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after its
shelf life.
When if medium or reagent touched eyes or
mouth, immediately wash with plenty of water,
and consult a physician.
Any medium, reagent and materials must be
sterilized by autoclaving or boiling water after use,
and then disposed as industrial waste according to
the law or regulations related to dispose.