流式细胞仪用微球

来源：seebio.cn作者：西宝生物人气：-发表时间：2013-07-29 14:25:00【大中小】

流式细胞仪是对细胞进行自动分析和分选的装置。它可以快速测量、存贮、显示悬浮在液体中的分散细胞的一系列重要的生物物理、生物化学方面的特征参量，并可以根据预选的参量范围把指定的细胞亚群从中分选出来。多数流式细胞计是一种零分辨率的仪器，它只能测量一个细胞的诸如总核酸量，总蛋白量等指标，而不能鉴别和测出某一特定部位的核酸或蛋白的多少。也就是说，它的细节分辨率为零。

流式细胞仪应用：1.分析细胞表面标志、2.分析细胞内抗原物质、3.分析细胞受体、4.分析肿瘤细胞的DNA、RNA含量、5.分析免疫细胞的功能。

标准化、校准、对照是日常质控的几个不可缺的方面。流式细胞仪可靠地、重复检测细胞。但这些应用要求流式细胞仪进行校准。一般这些校准都依靠标准微球来完成。微球有各种规格用于校准大小、荧光强度、荧光补偿、绝对计数和激光器校准。荧光微球检测结果可以从荧光强度转化成为MESF值或ABC值。这些标准数据可用于不同仪器间或不同实验室间的质控对照。此外流式细胞仪灵敏度也可以作为仪器质控的常规检测。

西宝生物提供流式细胞仪所用微球如下：

货号	英文品名	中文品名	固含量	粒径	规格
AEG0250A	AccuCount Rainbow Fluorescent Particles	绝对计数彩虹荧光微球	106/mL	8.0-12.9μm	3mL
AEG0251A	AccuCount Ultra Rainbow Fluorescent Particles	绝对计数超级彩虹荧光微球	106/mL	5.0-5.9μm	10mL
AEG0252A	AccuCount Blank Particles	绝对计数空白微球	106/mL	8.0-12.9μm	10mL
AEG0252B	AccuCount Blank Particles	绝对计数空白微球	106/mL	13.0-17.9μm	10mL
AEG0252C	AccuCount Blank Particles	绝对计数空白微球	106/mL	2.0-2.4μm	10mL
AEG0252D	AccuCount Blank Particles	绝对计数空白微球	106/mL	5.0-5.9μm	10mL
AEG0252E	AccuCount Blank Particles	绝对计数空白微球	106/mL	7.0-7.9μm	10mL
AEG0253A	AccuCount Fluorescent Particles	绝对计数荧光微球	106/mL	8.0-12.9μm	3mL
AEG0253B	AccuCount Fluorescent Particles	绝对计数荧光微球	106/mL	5.0-5.9μm	5mL
AEG0253C	AccuCount Fluorescent Particles	绝对计数荧光微球	106/mL	7.0-7.9μm	10mL
AEG0253C	AccuCount Fluorescent Particles	绝对计数荧光微球	106/mL	7.0-7.9μm	5mL
AEG0552A	AccuCount Fluorescent Particles Kit	绝对计数荧光微球试剂盒	4 bottles, 0.5x106/mL, 1.0x106/mL, 0.5x107/mL, & 1.0x107/mL,	5.0-5.9μm	4x1mL
AEG0558A	Streptavidin Blue Particle Array Kit, 10 peaks	链霉亲和素蓝色微球阵列试剂盒	106/mL	5.0-5.9μm	10x1mL
AEG0559A	Streptavidin Blue Particle Array Kit, Odd # peaks	链霉亲和素蓝色微球阵列试剂盒	106/mL	5.0-5.9μm	5x1mL
AEG0594A	Streptavidin Blue Particle Array Kit, Even # peaks	链霉亲和素蓝色微球阵列试剂盒	106/mL	5.0-5.9μm	5x1mL
AEG0320A	Carboxyl Blue Particle Array Chemistry Development Particles	羧基蓝色微球阵列化学开发试剂盒	108/mL	5.0-5.9μm	2mL
AEG0560A	Carboxyl Blue Particle Array Kit, 7 peaks	羧基蓝色微球阵列试剂盒	108/mL	3.5-3.9μm	7x1mL
AEG0561A	Carboxyl Blue Particle Array Kit, 8 peaks	羧基蓝色微球阵列试剂盒	108/mL	4.0-4.9μm	8x1mL
AEG0583A	Carboxyl Blue Particle Array Kit, 10 peaks	羧基蓝色微球阵列试剂盒	108/mL	5.0-5.9μm	10x1mL
AEG0585A	Carboxyl Blue Particle Array Kit, Odd # peaks	羧基蓝色微球阵列试剂盒	108/mL	5.0-5.9μm	5x1mL
AEG0590A	Carboxyl Blue Particle Array Kit, Even # peaks	羧基蓝色微球阵列试剂盒	108/mL	5.0-5.9μm	5x1mL
AEG0595A	Carboxyl Blue Particle Array Kit, 9 peaks	羧基蓝色微球阵列试剂盒	107/mL	7.0-7.9μm	9x1mL
AEG0562A	Goat anti-Mouse IgG Blue Particle Array Kit, 10 peaks	羊抗小鼠IgG包被的蓝色微球阵列试剂盒	106/mL	5.0-5.9μm	10x1mL
AEG0563A	Goat anti-Mouse IgG Blue Particle Array Kit, Odd # peaks	羊抗小鼠IgG包被的蓝色微球阵列试剂盒	106/mL	5.0-5.9μm	5x1mL
AEG0596A	Goat anti-Mouse IgG Blue Particle Array Kit, Even # peaks	羊抗小鼠IgG包被的蓝色微球阵列试剂盒	106/mL	5.0-5.9μm	5x1mL
AEG0365A	Goat anti-Mouse IgG (Fc) Coated Fluorescent Particles, Blue	羊抗小鼠IgG包被的蓝色荧光微球	0.1% w/v	2.5-4.5μm	5mL
AEG0581A	Amino Blue Particle Array Kit, 7 peaks	氨基蓝色微球阵列试剂盒	108/mL	3.5-3.9μm	7x1mL
AEG0586A	Pink Fluorescent Particle Array Kit,9 peaks	粉色荧光微球阵列试剂盒	108/mL	3.0-3.4μm	9x1mL
AEG0592A	Pink Fluorescent Particle Array Kit, 10 peaks	粉色荧光微球阵列试剂盒	108/mL	4.0-4.5μm	10x1mL
AEG0556A	Blue Particle Array Kit, 7 peaks	蓝色微球阵列试剂盒	108/mL	3.5-3.9μm	7x1mL
AEG0557A	Blue Particle Array Kit, 8 peaks	蓝色微球阵列试剂盒	108/mL	4.0-4.9μm	8x1mL
AEG0582A	Blue Particle Array Kit, 10 peaks	蓝色微球阵列试剂盒	108/mL	5.0-5.9μm	10x1mL
AEG0584A	Blue Particle Array Kit, Odd # peaks	蓝色微球阵列试剂盒	108/mL	5.0-5.9μm	5x1mL
AEG0588A	Blue Particle Array Kit, Even # peaks	蓝色微球阵列试剂盒	108/mL	5.0-5.9μm	5x1mL
AEG0593A	Blue Particle Array Kit. 9 peaks	蓝色微球阵列试剂盒	107/mL	7.0-7.9μm	9x1mL
AEG0167A	Rainbow Alignment Particles	彩虹线性微球	1.2x106/mL	3.5-3.9 um	5mL
AEG0168A	Rainbow Linear Calibration Particles	彩虹线性校准荧光微球	107/mL	3.0-3.4μm	5mLFor use in Linear Scale
AEG0175A	Rainbow Fluorescent Particles	彩虹荧光微球	107/mL	8.1-12.0μm	2mL
AEG0175B	Rainbow Fluorescent Particles	彩虹荧光微球	107/mL	1.8-2.2μm	5mL
AEG0175C	Rainbow Fluorescent Particles	彩虹荧光微球	107/mL	3.0-3.4μm	5mL
AEG0175D	Rainbow Fluorescent Particles	彩虹荧光微球	107/mL	3.0-3.4μm	5mL
AEG0175E	Rainbow Fluorescent Particles	彩虹荧光微球	107/mL	3.5-4.0μm	5mL
AEG0175F	Rainbow Fluorescent Particles	彩虹荧光微球	107/mL	5.0-5.9μm	5mL
AEG0175G	Rainbow Fluorescent Particles	彩虹荧光微球	107/mL	6.0-6.4μm	5mL
AEG0175H	Rainbow Fluorescent Particles	彩虹荧光微球	107/mL	6.5-8.0μm	5mL
AEG0180A	UltraRainbow Fluorescent Particles	超彩虹荧光微球	106/mL	3.0-3.4μm	10x3mL
AEG0180B	UltraRainbow Fluorescent Particles	超彩虹荧光微球	106/mL	3.0-3.4μm	2x15mL
AEG0180C	UltraRainbow Fluorescent Particles	超彩虹荧光微球	107/mL	8.1-12.0μm	2mL
AEG0180D	UltraRainbow Fluorescent Particles	超彩虹荧光微球	107/mL	1.0-1.4μm	5mL
AEG0180E	UltraRainbow Fluorescent Particles	超彩虹荧光微球	5x106/mL	13.0-17.9μm	2mL
AEG0180F	UltraRainbow Fluorescent Particles	超彩虹荧光微球	1% w/v	18.0-24.9μm	5mL
AEG0180F	UltraRainbow Fluorescent Particles	超彩虹荧光微球	1% w/v	25.0-35.0μm	5mL
AEG0180G	UltraRainbow Fluorescent Particles	超彩虹荧光微球	107/mL	3.0-3.4μm	2mL
AEG0543A	UltraRainbow Fluorescent Particles	超彩虹荧光微球	107/mL	3.0-3.4μm	20mL
AEG0591A	UltraRainbow Fluorescent Particles	超彩虹荧光微球	107/mL	3.5-3.9μm	2mL
AEG0377A	Fluorescent Alignment Particles	荧光线性微球	108/mL	3.0-3.4μm	5mL
AEG0549A	Rainbow QC Kit, includes BCP-60-2, RFP-60-2, RCP-30-2H, and RCP-30-2L,	彩虹QC试剂盒	107/mL	3.0-3.4μm	4x2mL
AEG0545A	Rainbow Calibration Kit,includes RCP-30-5A(Peak 1), RCP-30-5A(Peak 4) and RCP-30-5A(Peak 8)	彩虹校准试剂盒	107/mL	3.0-3.4μm	3x5mL
AEG0169A	Rainbow Calibration Particles, 4 peaks	彩虹校准微球	107/mL	1.8-2.2μm	5mL
AEG0170A	Rainbow Calibration Particles, 6 peaks	彩虹校准微球	107/mL	3.0-3.4μm	5mL
AEG0171A	Rainbow Calibration Particles, 8 peaks	彩虹校准微球	107/mL	3.0-3.4μm	5mL
AEG0172A	Rainbow Calibration Particles	彩虹校准微球	107/mL	3.2 (+/- 0.1) um	5mL
AEG0173A	Rainbow Calibration Particles, 4 peaks	彩虹校准微球	107/mL	3.5-4.0μm	5mL
AEG0174A	Rainbow Calibration Particles, 6 peaks	彩虹校准微球	107/mL	6.0-6.4μm	5mL
AEG0177A	UltraRainbow Calibration Kit, 3 peaks	超彩虹校准试剂盒	3.0-3.4 um	106/mL	10x3mL
AEG0178A	UltraRainbow Calibration Particles Kit, 6 peaks	超彩虹校准试剂盒	107/mL	3.5-3.9μm	1x3mL (blank) & 1x2mL (fluorescent)
AEG0178B	UltraRainbow Calibration Particles Kit, 6 peaks	超彩虹校准试剂盒	107/mL	5.0-5.4μm	1x3mL (blank) & 1x2mL (fluorescent)
AEG0114A	EasyComp Fluorescent Particles, Blank	EasyComp空白荧光微球	107/mL	3.0-3.4μm	5mL
AEG0553A	FITC Calibration Particles, 5 peaks	FITC标记校准微球	2x106/mL	3.0-3.4μm	5x1mL
AEG0554A	PE-Cy5 Calibration Particles, 5 peaks	PE-Cy5 校准微球	2x106/mL	3.0-3.4μm	5x1mL
AEG0555A	PE Calibration Particles, 5 peaks	PE校准微球	2x106/mL	3.0-3.4μm	5x1mL
AEG0542A	Allophycocyanin Calibration Particles, 6 peaks	别藻蓝蛋白校准微球	107/mL	3.0-3.4μm	2x5mL
AEG0179A	UltraRainbow Calibration Particles	超彩虹校准微球	8.0 - 12.9 um	107/mL	2mL
AEG0550A	Nano Polystyrene Size Standard Kit, Analytical Grade,	黄色纳米荧光标准试剂盒，分析级别	106/mL,	0.1-0.3μm、 0.4-0.6μm 、0.7-0.9μm 、&1.0-1.9μm	4x5mL
AEG0551A	Nano Fluorescent Size Standard Kit, Flow Cytometry Grade, Yellow,	黄色纳米荧光标准试剂盒，流式细胞级别	106/mL,	0.1-0.3μm 0.4-0.6μm 0.7-0.9μm &1.0-1.9μm	4x5mL
AEG0243A	Yellow Calibration Particles, 6 peak	黄色校准微球	107/mL	6.0-8.0μm	5mL
AEG0568A	Polystyrene Particle Size Standard Kit, Flow Cytometry Grade,	聚苯乙烯微球粒径标准试剂盒，流式细胞级别	2.5x106/mL,	2.0μm 3.3μm 5.05μm 7.66μm 10.2μm 15.3μm	6x5mL
AEG0256A	Blank Calibration Particles	空白校准微球	5x106/mL	8.0-12.9μm	5mL
AEG0256B	Blank Calibration Particles	空白校准微球	107/mL	1.0-1.4μm	5mL
AEG0256C	Blank Calibration Particles	空白校准微球	5x106/mL	13.0-17.9μm	5mL
AEG0256D	Blank Calibration Particles	空白校准微球	5x105/mL	18.0-24.9μm	5mL
AEG0256E	Blank Calibration Particles	空白校准微球	107/mL	1.8-2.2μm	5mL
AEG0256F	Blank Calibration Particles	空白校准微球	0.5x106/mL	25.0-35.0μm	5mL
AEG0256G	Blank Calibration Particles	空白校准微球	107/mL	3.0-3.4μm	5mL
AEG0256H	Blank Calibration Particles	空白校准微球	107/mL	3.2 (+/-0.1μm)	5mL
AEG0256I	Blank Calibration Particles	空白校准微球	107/mL	3.5-4.0μm	5mL
AEG0256J	Blank Calibration Particles	空白校准微球	107/mL	5.0-5.9μm	5mL
AEG0256K	Blank Calibration Particles	空白校准微球	107/mL	6.0-6.4μm	5mL
AEG0256L	Blank Calibration Particles	空白校准微球	107/mL	6.5-8.0μm	5mL
AEG0378A	Fluorescent Calibration Particles, 800nm EM, 4 peaks	荧光校准微球	107/mL	3.5μm (+/- 0.2μm)	2mL
AEG0375A	Drop Delay Calibration Particles	液滴延迟校准微球	108/mL	6.0-8.0μm	2mL
AEG0131A	EasyComp Fluorescent Particle Kit (Blank, FITC, PE & PE-Cy5), 4 vials,	EasyComp荧光微球试剂盒	107/mL	3.0-3.4μm	1x5mL & 3x1mL
AEG0132A	EasyComp Fluorescent Particle Kit (Blank,FITC,PE, PE-Cy5 & APC), 5 vials,	EasyComp荧光微球试剂盒	107/mL	3.0-3.4μm	1x5mL & 4x1mL
AEG0133A	EasyComp Fluorescent Particle Kit (Blank,FITC,PE, PE-TR & PE-Cy5), 5 vials,	EasyComp荧光微球试剂盒	107/mL	3.0-3.4μm	1x5mL & 4x1mL
AEG0134A	EasyComp Fluorescent Particle Kit (Blank, FITC, PE, PE-TR, PE-Cy5, PE-Cy7, APC & APC-Cy7), 8 vials,	EasyComp荧光微球试剂盒	107/mL	3.0-3.4μm	1x5mL & 7x1mL
AEG0135A	EasyComp Fluorescent Particle Kit (Blank, FITC, PE, PE-TR, PE-Cy5, APC & APC-Cy7), 7 vials,	EasyComp荧光微球试剂盒	107/mL	3.0-3.4μm	1x5mL & 6x1mL
AEG0126A	EasyComp Fluorescent Particles, APC-Cy7	APC-Cy7标记的 EasyComp荧光微球	107/mL	3.0-3.4μm	1mL
AEG0127A	EasyComp Fluorescent Particles, APC	APC标记的 EasyComp荧光微球	107/mL	3.0-3.4μm	1mL
AEG0136A	EasyComp Fluorescent Particles, FITC	FITC标记的 EasyComp荧光微球	107/mL	3.0-3.4μm	1mL
AEG0145A	EasyComp Fluorescent Particles, PE-Cy5	PE-Cy5标记的 EasyComp荧光微球	107/mL	3.0-3.4μm	1mL
AEG0146A	EasyComp Fluorescent Particles, PE-Cy7	PE-Cy7标记的 EasyComp荧光微球	107/mL	3.0-3.4μm	1mL
AEG0147A	EasyComp Fluorescent Particles, PE-TR	PE-TR标记的 EasyComp荧光微球	107/mL	3.0-3.4μm	1mL
AEG0148A	EasyComp Fluorescent Particles, PE	PE标记的 EasyComp荧光微球	107/mL	3.0-3.4μm	1mL
AEG0594A	Streptavidin Blue Particle Array Kit, 10 peaks	链霉亲和素蓝色微球阵列试剂盒	106/mL	5.0-5.9μm	10x1mL
AEG0558A	Streptavidin Blue Particle Array Kit, Odd # peaks	链霉亲和素蓝色微球阵列试剂盒	106/mL	5.0-5.9μm	5x1mL
AEG0559A	Streptavidin Blue Particle Array Kit, Even # peaks	链霉亲和素蓝色微球阵列试剂盒	106/mL	5.0-5.9μm	5x1mL
AEG0320A	Carboxyl Blue Particle Array Chemistry Development Particles	羧基蓝色微球阵列化学开发试剂盒	108/mL	5.0-5.9μm	2mL
AEG0560A	Carboxyl Blue Particle Array Kit, 7 peaks	羧基蓝色微球阵列试剂盒	108/mL	3.5-3.9μm	7x1mL
AEG0561A	Carboxyl Blue Particle Array Kit, 8 peaks	羧基蓝色微球阵列试剂盒	108/mL	4.0-4.9μm	8x1mL
AEG0583A	Carboxyl Blue Particle Array Kit, 10 peaks	羧基蓝色微球阵列试剂盒	108/mL	5.0-5.9μm	10x1mL
AEG0585A	Carboxyl Blue Particle Array Kit, Odd # peaks	羧基蓝色微球阵列试剂盒	108/mL	5.0-5.9μm	5x1mL
AEG0590A	Carboxyl Blue Particle Array Kit, Even # peaks	羧基蓝色微球阵列试剂盒	108/mL	5.0-5.9μm	5x1mL
AEG0595A	Carboxyl Blue Particle Array Kit, 9 peaks	羧基蓝色微球阵列试剂盒	107/mL	7.0-7.9μm	9x1mL
AEG0562A	Goat anti-Mouse IgG Blue Particle Array Kit, 10 peaks	羊抗小鼠IgG包被的蓝色微球阵列试剂盒	106/mL	5.0-5.9μm	10x1mL
AEG0563A	Goat anti-Mouse IgG Blue Particle Array Kit, Odd # peaks	羊抗小鼠IgG包被的蓝色微球阵列试剂盒	106/mL	5.0-5.9μm	5x1mL
AEG0596A	Goat anti-Mouse IgG Blue Particle Array Kit, Even # peaks	羊抗小鼠IgG包被的蓝色微球阵列试剂盒	106/mL	5.0-5.9μm	5x1mL
AEG0365A	Goat anti-Mouse IgG (Fc) Coated Fluorescent Particles, Blue	羊抗小鼠IgG包被的蓝色荧光微球	0.1% w/v	2.5-4.5μm	5mL
AEG0392A	Fluorescent Particle Slide, UV	UV荧光微球切片		10.0-14.0μm
AEG0393A	Fluorescent Particle Slide, Yellow	黄色荧光微球切片		10.0-14.0μm
AEG0394A	Fluorescent Particle Slide,NileRed	尼罗红色荧光微球切片		10.0-14.0μm
AEG0395A	Fluorescent Particle Slide, Purple	紫色荧光微球切片		10.0-14.0μm
AEG0396A	Fluorescent Particle Slide, Sky Blue	天蓝色荧光微球切片		10.0-14.0μm
AEG0397A	Fluorescent Particle Slide, 4 different fluorescent beads; UV, Yellow, Nile Red and Blue	荧光微球切片套装		10.0-14.0μm
AEG0398A	Fluorescent Particle Slide, 4 different fluorescent beads; UV, Yellow, Nile Red and Purple	荧光微球切片套装		10.0-14.0μm
AEG0399A	Fluorescent Particle Slide, UV	UV荧光微球切片		5.0-5.9μm
AEG0400A	Fluorescent Particle Slide, Yellow	黄色荧光微球切片		5.0-5.9μm
AEG0401A	Fluorescent Particle Slide,NileRed	尼罗红色荧光微球切片		5.0-5.9μm
AEG0402A	Fluorescent Particle Slide, Sky Blue	天蓝色荧光微球切片		5.0-5.9μm
AEG0403A	Mirror Slide, each	Mirror Slide, each
AEG0176A	Rainbow Fluorescent Particle Slide	彩虹荧光微球切片		10.0-14.0μm
AEG0176B	Rainbow Fluorescent Particle Slide	彩虹荧光微球切片		13.0-17.9μm
AEG0176C	Rainbow Fluorescent Particle Slide	彩虹荧光微球切片		13.0-17.9μm	Level 5 Intensity
AEG0176D	Rainbow Fluorescent Particle Slide	彩虹荧光微球切片	3.0-3.4μm	5 Intensities	For use in linear scale
AEG0176E	Rainbow Fluorescent Particle Slide	彩虹荧光微球切片	3.0-3.4μm	5 Intensities in individual wells	For use in linear scale
AEG0176E	Rainbow Fluorescent Particle Slide	彩虹荧光微球切片	3.0-3.4μm	5 Intensities in individual wells	For use in linear scale
AEG0176F	Rainbow Fluorescent Particle Slide	彩虹荧光微球切片		3.0-3.4μm	6 IntensitiesFor use in log scale
AEG0176G	Rainbow Fluorescent Particle Slide	彩虹荧光微球切片		5.0-5.9μm
AEG0176H	Rainbow Fluorescent Particle Slide	彩虹荧光微球切片	6.0-6.4μm	3 Intensities
AEG0176I	Rainbow Fluorescent Particle Slide	彩虹荧光微球切片		0.5-15.0μm	6 Sizes