西宝生物提供广泛用于各类酶底物、分子生物学、转基因、人造AMC-肽酶底物、蛋白质的膜分离/分子生物学、ELISA检测/免疫学、食品微生物学、环境微生物学、植物生长激素等原料试剂，欢迎垂询！

转基因烟草、树苗，在camv-35s推动下表达gus(β-葡（萄）糖苷酶)。这些树苗在37℃培养，加入1mg/ml的X-glcUA, MagentaTM-glcUA,或SalmonTM-glcUA时，可以保持性能。

Gus reporter system:
Transgenic tobacco seedlings, eXpressing gus (beta-Glucuronidase) under the control of the CaMV-35S promoter. The seedlinqs were stained using standard conditions with vacuum infiltration of either X-glcUA, MagentaTM-glcUA, or SalmonTM-glcUA at l mg per ml, with overnight incubation at 37℃.

Enzyme substrates: （各类酶底物）
B-7300 X-β-D-glcUA·CHX 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7350 MagentaTM -β-D-glcUA·CHX 5-Bromo-6-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7400 X-β-D-glcUA·Na 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt
B-8902 LaposTM -β-D-glcUA·CHX 5-Bromo-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
C-5050 SalmonTM -β-D-glcUA·CHX 6-Bromo-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
I-6100 YTM -β-D-glcUA·CHX 3-Indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
I-6140 YTM -β-D-glcUA·Na 3-Indoxyl-beta-D-glucuronic acid, sodium salt
M-5700 MUG, 4-MU-β-D-glcUA 4-Methylumbelliferyl- beta-D-glucuronic acid
N-4450 PNP-β-D-glcUA 4-Nitrophenyl- beta-D-glucuronic acid
N-4465 PNP-β-D-glcUA·Na 4-Nitrophenyl- beta-D-glucuronic acid, sodium salt
P-2480 Phenolphthalein-β-D-glcUA Phenolphthalein- beta-D-glucuronic acid
P-4150 Phenyl-β-D-glcUA Phenyl- beta-D-glucuronic acid

Plant growth regulators: （植物生长激素）
G-2700 G A3 Gibberellic acid
I-1000 IAA Indole-3-acetic acid
I-2000 IBA Indole-3-butyric acid
K-4000 Kinetin
Z-3000 Trans-Zetain Riboside

Iacz基因作为一种通讯基因在微生物分子生物学中被频繁使用，Iacz编译一种能够水解底物X-β-D-gal，形成一种靛蓝染料沉淀，很多常见的无性繁殖的带菌者在DNA片断插入复制时都有Iacz基因被表达DNA片断插入Iacz片断时，通常阻碍Iacz片断工作，只有一小部分Iacz基因片断用于保持质粒体大小。Iacz基因超过3000个碱基，而且大部分复制质粒体比它还小，这些小片断叫作α区，位于Iacz基因起始端，α区基因可以通过基因互补来控制β葡萄糖苷酶的活性，所以细胞中Iacz基因被检测出α区变异。β葡萄糖苷酶的α片断和形成活性β葡萄糖苷酶的变异的α片断相第影响，那此被无性繁殖的带菌都插入的细菌可以被真实的检出。蓝色菌落表示α互补，白色或无色就可能是有克隆片断插入Iacz基因。 IPTG通过阻断Iac增强了β-葡（萄）糖苷酶的活性。
培养基和生长条件：LB琼脂平板，加入100mg/ml X-β-D-gal， 100mg/ml IPTG 37℃培养24小时。

LacZ reporter gene system
The lacZ gene is frequently used in molecular biology as a reporter gene. LacZ encodes the enzyme beta-Galactosidase which can hydrolyze the substrate X-β-D-gal (5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside, B-7150) to form a precipitating blue indigo dye. Many popular cloninq vectors have part of the lacZ gene to indicate when DNA fragments are cloned (inserted) into them. The DNA fragment, tLen inserted into the lacZ fragment, usually inactivates (blocks) the lacZ gene fragment from working. Qnly a Small fragment of the lacZ gene is used in order to keep the size of the plasmid small. (The IacZ gene is over 3000 base pairs long, and most cloninq plasmids are smaller than that.) This small fragment is called the alpha region and is located in the beginning ot the lacZ gene. The alpha portion of the gene can result in beta-Galactosidase enzyme activity by a process called “intragenic complemen-tation” whereby the rest of the lacZ gene is provided in the cell by an alpha mutant of the lacZ gene.The alpha fragment of the beta-Galactosidase enzyme interacts with the complementary portion of the enzyme provided by the alpha mutant to form an active beta-Galactosidase enzyme. Therefore,bacterial colonies can be visually screened to determine which colonies have the cloning vector inserted. Blue colonies show alpha complemen-tation, While white or colorless colonies probably have the cloned fragment inserted within the lacZ gene.
IPTG (Isopropyl-beta-D-thiogalactopyranoside, I-8000) enhances beta-Galactosildase activity by blocking the lac repressor. (by David Demirjian and Tom Jung)
Culture media and growth conditions
Luria-Bertani (LB) agar plates with 100 μg per ml of X-β-D-gal (5-Bromo-4-chloro-3-indoxyl-beta-D- galactopyranoside, B-7150), 100μg per ml of lPTG (lsopropyl-beta-D-thiogalactopyranoside, I-8000). Plates were incubated for 24 hours at 37℃. (Courtesy of Dr. Lawrence Restaino)

Substrates for beta-Galactosidase:
B-7150 X-gal, X-β-D-gal 5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside
B-7200 MagentaTM -β-D-gal 5-Bromo-6-chloro-3-indoxyl-beta-D-galactopyranoside
B-8900 LapisTM -β-D-gal 5-Bromo-3-indoxyl-beta-D-galactopyranoside
C-5000 SalmonTM -β-D-gal 6-Chloro-3- indoxyl-beta-D-galactopyranoside
F-5500 F-β-D-gal 6-Fluoro-3- indoxyl-beta-D-galactopyranoside
I-6000 YTM -β-D-gal 3-Indoxyl- beta-D-galactopyranoside
I-7600 Iodo-β-D-gal 5-Iodo-3- indoxyl- beta-D-galactopyranoside
M-4100 GreenTM -β-D-gal N-Methylindoxyl- beta-D-galactopyranoside

Inducer:
I-8000 IPTG Isopropyl- beta-D-galactopyranoside

被Luc因子表达的荧光素酶的特殊细胞将表现出荧光。
The presence of Iuciferase expressed by the Iuc gene is indicated by a glow or flash emitted form the particular well as illustrated above.

S products:
L-8200 D-Luciferin Firefly, free acid (synth.)
L-8220 D-Luciferin Firefly, potassium salt (synth.)
L-8240 D-Luciferin Firefly, sodium salt (synth.)

清洁剂/表面活性剂
以下的表面活性剂被广泛的应用于生化分离提纯和化验，这些表面活性剂在离析和提纯膜蛋白和其他分子蛋白时是特别有用的。

Detergents/Surtactants
The following detergents can be used in a variety of biochemical separations, purifications, and assays. These surfactants are especially useful in the isolation and purification of membrane proteins and ther cellular proteins.

C-5910 Cholic acid, sodium salt
D-0280 n-Decyl-beta-D-glucopyranoside
D-0290 n-Decyl-4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside
D-3100 Digitonin UDP24
D-3200 Digitonin special grade (Water soluble)
D-8820 n-Dodecyl-beta-D-glucopyranoside
D-8823 n-Dodecyl-beta-D-maltoside
D-8824 n-Dodecyl-d25-beta-D-maltoside
E-8900 Ethyl-beta-D-thiogalactopyranoside
H-1750 n-Heptyl-beta-D-glucopyranoside
H-1758 n-Heptyl-beta-D-thioglucopyranoside
H-1770 n-Hexyl-beta-D-glucopyranoside
N-8400 n-Nonyl-beta-D- glucopyranoside
O-2000 n-Octyl- beta-D- glucopyranoside
O-2700 n-Octyl- beta-D- thiogalactopyranoside
O-2710 n-Octyl-beta-D-thioglucopyranoside
S-6500 Sodium taurocholate
S-6560 Sodium taurodeoxychloate
S-6600 Sodium tauroglycocholate
Numerous permutations o different sugars, thiosugars, and chain lengths are possible. Please inquire for your custom made detergents/surfactants.

提供很多种酶联免疫检测的重要底物，可溶的、可溶显色的、荧光的一样非常重要的底物。

免疫学
Zymosan A是一种不可溶的酵母的细胞壁，主要有蛋白质，碳水化合物组合而成，能够用于阻止补足物的活动。
最大的Zymosan A的生产商，生产很多公斤级原料，每一批都检测其生物活性。 其他的Zymosans 请询价

SEEBIO’s products:
Horseradish Peroxidase:（辣根过氧化物酶）
D-2000 HDCBS·Na 3,5-Dichloro-2-hydroxybenzenesulfonic acid, sodium salt
D-2050 HDCBS·diNa 3,5-Dichloro-2-hydroxybenzenesulfonic acid, disodium salt
T-2100 TMB 3,3’,5,5’-Tetramethylbenzidine, free base
T-2150 TMB·diHCl 3,3’,5,5’-Tetramethylbenzidine dihydrochloride
Soluble substrates for Alkaline Phosphatase:（碱性磷酸酶的可溶性底物）
M-5800 4-MU-phos 4-Methylumbelliferyl phosphate, free acid
M-550 4-MU-phos·diAMPD 4-Methylumbelliferyl phosphate, di(2-amino-2-methyl-1,3-propanediol) salt
M-5900 4-MU-phos·diCHX 4-Methylumbelliferyl phosphate, dicyclohexylammonium salt
M-5950 4-MU-phos·diNa 4-Methylumbelliferyl phosphate, disodium salt
N-4700 PNP- phos·diCHX 4-Nitrophenyl phosphate, dicyclohexylammonium salt
N-5000 PNP- phos·diNa 4-Nitrophenyl phosphate, disodium salt

Zymosan A
Zymosan A (Z-9500) is an insoluble cell wall of yeast mainly consisting of protein carbohydrate complexes wich can be used to inactivate complements. (Prepared according to the procedure of Pillemer, et. al., J. Exp. Med. 103:1 (1956).)
SEEBIO Is the largest manufacturer of Zymosan A using proprietary technology producing multi-kilogram lots of the material. Each lot is tested for its biological actilvity.
Please inquire also for other Zymosans.

E. coli O157:H7（病原性大肠杆菌）是一种危险的损伤E. coli 时常存在于汉堡，鸡肉和其他肉食品中。根据USDA大概10000人每年因为不熟的包含着E. coli O157:H7的肉制品而染病，这个危险的有机体能够引起很多疾病甚至死亡。

培养基和生长条件： 麦克拉肯山梨（糖）醇 琼脂平盘含100ug/ml X-glcUA·Na(BClG), 35℃过液，大部分的含β-葡（萄）糖苷酸酶的E. coli会和酶底物X-glcUA·Na反应成为蓝色菌落，没有β-葡（萄）糖苷酸酶的大肠杆菌为无色状态。

Testing for E. coli O157:H7
E. coli O157:H7 is a dangerous strain of E.coli which is sometimes present in hamburger, chicken, and other meat products, According to the USDA, as many as 10,000 people each year become sick from eating undercooked meat containing E. coli O157:H7. This dangerous organism can cause severe illness and even death.
Culture media and growth conditions
MacConkey Sorbitol Agar Plates with 100 micrograms per ml of X-glcUA·Na (also known as BClG). Plates were incubated overnight at 35℃. Most E. coli contain the enzyme beta-Glucuronidase, which reacts with the enzyme substrate X-glcUA·Na to produce the deep blue seen in the colonies. E. coli O157:H7 lacks the beta-Glucuronidase enZyme, and the colonies remain basically colorless. (Courtesy of Dr. Lawrence Restaino)

SEEBIO’s products:
B-7300 X-β-D-glcUA·CHX 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7350 MagentaTM -β-D- glcUA·CHX 5-Bromo-6-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7400 X-β-D-glcUA·Na 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt
B-8902 LapisTM -β-D- glcUA·CHX 5-Bromo-3-indoxyl- beta-D-glucuronic acid, cyclohexylammonium salt
C-5050 SalmonTM -β-D- glcUA·CHX 6-Chloro-3- indoxyl- beta-D-glucuronic acid, cyclohexylammonium salt
I-6000 YTM -β-D- glcUA·CHX 3-Indoxyl- beta-D- glucuronic acid, cyclohexylammonium salt
I-6140 YTM -β-D- glcUA·Na 3-Indoxyl- beta-D- glucuronic acid, sodium salt
M-5700 MUG,4-MU -β-D-glcUA 4-Methylumbelliferyl- beta-D- glucuronic acid

BCM O 157:H7(+)
l Plates stable for over 60 days
l False negatives are raw
l More specitic than other plating medium
l Isolates over 2 times more E. coli O 157:H7 than standard USDA plating medium
These data are confirmed by independent investigations and through governmental evaluations as well as publications in USA, Europe and Japan

平板保持稳定60天以上
自然阴性状态
比其它平板培养基更多的规格
比标准USDA底盘培养基多2次分离E. coli O 157:H7（病原性大肠杆菌）
这些数据是通过和美国，欧洲，日本之类的政府的评价和独立的调查得出的结论

SEEBIO’s product:
C-0500 BCM O 157:H7(+) Plating Medium
Please ask for more information, including the protocol and literature.

BCM Listeria monocytogenes Detection System
l The best available test system with a fluorogenic selective enrichment broth and a chromogenic plating medium
l Confirmation within 6 to 24 hours after isolation
l The pre-enrichment is selective and repairs injured cells
l 2.5 times more Listeria monocytogenes isolated than the conventional USDA method
l The selective enrichment broth is fluorogenic and thus can be applied in an automated equipment
l Potential false positives are eliminated sequentially by the system’s selectivity

BCM 单包李斯特菌的探测体系：
一种最可行的用荧光选择探测的体系，金色平板
分离后6-24小时内可确认
选择性的预浓缩并且修复受损细胞
比常规的USDA方法多分离2.5倍多的单包李斯特菌
选择性的浓缩肉汤含荧光的，也可用于自动化操作
人造的（正电压）阳离子被这个体系选择性的持续的消除

SEEBIO’s product:
C-0600 BCM Listeria monocytogenes Detection System
Please ask for more information, including the protocol and literature

BCM Bacillus cereus and Bacillus thuringiensis
l A new fascinating test isolating both important organisms within 24 hours
l Other Bacillus species either form white colonies or do not grow
l There is no equivalent test on the market

BCM 蜡状芽孢杆菌和苏云金芽孢杆菌
一种新的可以在24小时内分离重要生物体的神奇的方法
其他杆菌种类为白色菌落或不生长
在市场上没有同类产品（方法）

SEEBIO’s product:
C-0700 BCM Bacillus cereus and Bacillus thuringiensis
Please ask for more information, including the protocol and literature.

ATP化验 ATP和虫荧光素就是萤火虫当闪动的时候有细菌存在就有ATP存在。在虫荧光酶催化下，ATP和萤火虫的荧光素反应，发出荧光。荧光的大小和样品中ATP含量成正比，荧光的测验非常的敏感，因此可以快速准确得出样品是否被细菌污染。

ATP occurs wherever bacteria are present. Reaction of ATP with Luciferin Firetly in the presence of Luciferase results in the emission of light as a glow or flash. The amount of light emitted is directly proportional to the amoumt of ATP present in the sample. The measurement of the emitted light is very sensitive thus allowing quick and accurate conclusions of the bacterial contamination of the sample.

SEEBIO’s product:
L-8200 D-Luciferin Firefly, free acid (synth.)
L-8220 D-Luciferin Firefly, potassium salt (synth.)
L-8240 D-Luciferin Firefly, sodium salt (synth.)
L-8080 Luciferase, American Firefly, natural

水和土壤的测试
SEEBIO为微生物鉴定提供一系列的酶底物环境筛选测试，包括β-葡萄糖苷酶和水中、土壤样本中细菌的β-葡萄糖苷酶的检测。

Water & Soils Testing
SEEBIO offers a wide range of enzyme substrates for the identification of microorganisms. Environ-mental screening tests most often involve the detection of beta-Glucuronidase and beta-Galactosidase positive bacteria in water and soil samples.

SEEBIO’s product:
Substrates for beta-Galactosidase:
A-7050 p-Aminophenyl-β-D-gal 4-Aminophenyl-beta-D-galactopyranoside
B-7150 X-gal, X-β-D-gal 5-Bromo-4-chloro-3-indoxyl-beta-D- galactopyranoside
B-7200 MagentaTM -β-D- gal 5-Bromo-6-chloro-3-indoxyl-beta-D- galactopyranoside
B-8900 LapisTM -β-D- gal 5-Bromo-3-indoxyl- beta-D- galactopyranoside
C-5000 SalmonTM -β-D- gal 6-Chloro-3- indoxyl- beta-D- galactopyranoside
F-5500 F-β-D- gal 6-Fluoro-3- indoxyl- beta-D- galactopyranoside
I-6000 YTM -β-D- gal 3-Indoxyl- beta-D- galactopyranoside
I-7600 Iodo-β-D- gal 5-Iodo-3- indoxyl- beta-D- galactopyranoside
M-4100 GreenTM -β-D- gal N-Methylindoxyl- beta-D- galactopyranoside
M-5550 4-MU -β-D-gal 4-Methylumbelliferyl- beta-D- galactopyranoside
N-4190 ONP-β-D- gal 2-Nitrophenyl- beta-D- galactopyranoside
N-4300 NPN-β-D- gal 4- Nitrophenyl- beta-D- galactopyranoside
P-4100 Phenyl -β-D-gal Phenyl- beta-D- galactopyranoside

Enhancer for beta-Galactosidase:
I-8000 IPTG Isopropyl-beta-D-thiogalactopyranoside

Substrates for beta-Galactosidase:
B-7300 X-β-D-glcUA·CHX 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7350 MagentaTM -β-D- glcUA·CHX 5-Bromo-6-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7400 X-β-D-glcUA·Na 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt
B-8902 LapisTM -β-D- glcUA·CHX 5-Bromo-3-indoxyl- beta-D-glucuronic acid, cyclohexylammonium salt
C-5050 SalmonTM -β-D- glcUA·CHX 6-Chloro-3- indoxyl- beta-D-glucuronic acid, cyclohexylammonium salt
I-6100 YTM -β-D- glcUA·CHX 3-Indoxyl- beta-D- glucuronic acid, cyclohexylammonium salt
I-6140 YTM -β-D- glcUA·Na 3-Indoxyl- beta-D- glucuronic acid, sodium salt
M-5700 MUG,4-MU -β-D-glcUA 4-Methylumbelliferyl- beta-D- glucuronic acid
N-4450 PNP-β-D-glcUA 4-Nitrophenyl- beta-D- glucuronic acid
N-4465 PNP-β-D-glcUA·Na 4-Nitrophenyl- beta-D- glucuronic acid, sodium salt
P-2480 Phenolphthalein-β-D-glcUA Phenolphthalein - beta-D- glucuronic acid
P-4150 Phenyl -β-D- glcUA Phenyl- beta-D- glucuronic acid

Enhancer for beta-Glucuronide:
M-3600 O-Me-glcUA·Na 1-O-Methyl- beta-D- glucuronic acid, sodium salt
I-8350 IPTGA Isopropyl - beta-D- thioglucuronic acid

AMC底物在特定酶反应时会发出荧光信号，这些底物是非常敏感的，而且他们非常适合自动化测试。
SEEBIO有高级的所有的实际合成新科技的发展，AMC派生物是必须的，因此，SEEBIO可以人工制造提取您所需要的AMC底物。
SEEBIO已经发展新的技术生产合成任何被需要的AMC衍生物，因此，SEEBIO也可以按您的特殊要求订做AMＣ底物。

Custom made AMC-Peptidase Substrates
AMC substrates (7-Amido-4-methylcoumarin substrates) give a soluble, fluorescent signal upon reaction with the correct enzyme. These substrates are extremely sensitive and are well extremely sensitive and are well suited to tests performed in automated instrumentation or robotics.
SEEBIO has developed proprietary new technology for synthesizing virtually any AMC derivative that might be required. Therefore, SEEBIO can custom manufacture the exact AMC substrate needed in your particular application.

SEEBIO’s standard AMC products:
A-1470 Ac-Lys-AMC·AcOH N-Acetyl-L-lysine 7-amido-4-methylcoumarin, acetate salt
A-4220 H-D-Ala-AMC·TFA D-Alanine 7- amido-4-methylcoumarin, trifluoroacetate salt
A-4500 H-Ala-AMC·TFA L- Alanine 7- amido-4-methylcoumarin, trifluoroacetate salt
A-8700 H-Arg-AMC·diHCl L-Arginine 7- amido-4-methylcoumarin dihydrochloride
A-8750 H-Arg-Arg-AMC·HCl L-Arginyl-L-arginine 7- amido-4-methylcoumarin hydrochloride
A-9300 H-Asp-AMC L-Aspartic acid alpha-(7- amido-4-methylcoumarin)
A-9305 H-Asp-AMC·TFA L-Aspartic acid alpha-(7- amido-4-methylcoumarin), trifluoroacetate salt
C-2100 N-α-CBZ- Arg-AMC·HCl N-alpha-CBZ-L-Arginine 7- amido-4-methylcoumarin hydrochloride
C-2128 CBZ-Lys-Lys- Arg-AMC·triAcOH N-CBZ-Lysyl-lysyl-arginine 7- amido-4-methylcoumarin, triacetate salt
C-2150 N-CBZ-Gly-Pro-AMC N-CBZ-Glycyl-L-proline 7- amido-4-methylcoumarin
C-6200 H-Cit- AMC·HBr L-Citrulline 7- amido-4-methylcoumarin hydrochloride
C-6300 H-Cit- AMC·TFA L-Citrulline 7- amido-4-methylcoumarin, trifluoroacetate salt
G-3845 H-Glu(OH)-AMC L-Glutamic acid alpha-(7- amido-4-methylcoumarin)
G-3850 H-Glu-AMC L-Glutamic acid gamma-(7- amido-4-methylcoumarin)
G-3950 Glutaryl-Gly-Arg-AMC Glutaryl-glycyl-L-arginine (7- amido-4-methylcoumarin)
G-4500 H-Gly- AMC·HBr Glycine 7- amido-4-methylcoumarin hydrochloride
G-6100 H-Gly-Gly-AMC·HCl Glycyl-glycine 7- amido-4-methylcoumarin hydrochloride
G-6300 Gly-Phe-AMC Glycyl-L-phenylalanine 7- amido-4-methylcoumarin
G-6350 Gly-Pro- AMC·HBr Glycyl-L-proline 7- amido-4-methylcoumarin hydrochloride
H-2350 H-His-AMC L-Histidine 7- amido-4-methylcoumarin
H-7300 H-Hyp-AMC L-Hydroxyproline 7- amido-4-methylcoumarin
I-7900 H-lle-AMC·TFA L-Isoleucine 7- amido-4-methylcoumarin, trifluoroacetate salt
L-2750 H-Leu-AMC L-Leucine 7- amido-4-methylcoumarin
L-2800 H-Leu- AMC·HCl L-Leucine 7- amido-4-methylcoumarin hydrochloride
L-9300 H-Lys- AMC·AcOH L-Lysine 7- amido-4-methylcoumarin, acetate salt
M-3170 H-Met- AMC·AcOH L-Methionine 7- amido-4-methylcoumarin, acetate salt
M-3200 H-Met- AMC·TFA L-Me thionine 7- amido-4-methylcoumarin, trifluoroacetate salt
O-3400 H-Orn-AMC·Cab L-Ornithine 7- amido-4-methylcoumarin, carbonate salt
P-3500 H-Phe- AMC·TFA L-Phenylalanine 7- amido-4-methylcoumarin, trifluoroacetate salt
P-7280 H-Pro- AMC·HBr L-Proline 7- amido-4-methylcoumarin hydrochloride
P-8500 H-Pyr-AMC L-Pyroglutamic acid 7- amido-4-methylcoumarin
S-2700 H-Ser- AMC·HCl L-Serine 7- amido-4-methylcoumarin hydrochloride
S-3300 H-Ser-Tyr-AMC L-Seryl-L-tyrosine 7- amido-4-methylcoumarin
T-8350 H-Trp-AMC L-Tryptophan 7- amido-4-methylcoumarin
T-8355 H-Trp- AMC·HCl L-Tryptophan 7- amido-4-methylcoumarin hydrochloride
T-9100 H-Tyr-AMC L-Tyrosine 7- amido-4-methylcoumarin
V-2000 H-Val-AMC L-Valine 7- amido-4-methylcoumarin
V-2100 H-Val- AMC·TFA L-Valine 7- amido-4-methylcoumarin, trifluoroacetate salt

Different salts and N-protected AMC derivatives for pptide synthesis are also available from SEEBIO. Please inquire for any AMC substrate that may be needed in your application.

酶的诱导剂和增强剂
SEEBIO提供一些既能诱导也能增强若干重要的酶／底物的反应的试剂，我们最新的很有效的诱导剂是Isopropyl-beta-D-thioglucuronic acid，sodiumsalt, Isopropyl-beta-D-thioglucuronic acid，sodiumsalt是一个不参与反应的诱导剂，他不会被酶水解，而且能够很有效的增强β-葡（萄）糖苷酸酶在大肠杆菌测试中敏感性。

Enzyme inducers and enhancers：
SEEBIO offers a number of reagents which either induce or enhance several important enzyme/substrate reactions. Our most recent and very effective inducer is Isopropyl-beta-D-thioglucuronic acid, sodium salt (I-8350).
Isopropyl-beta-D-thioglucuronic acid sodium salt is a “gratuitous” inducer, i.e. it is not hydrolyzed by the enzyme, and enhances very effectively the sensitivity of your beta-Glucuronidase test in E. coli by increasing the concentration of the enzyme.
The following compounds can also be used in conjumction with SEEBIO substrates to enhance color development:

alpha-Galactosidase M-3590 1-O-Methyl-alpha-D-galactopyranoside
beta-Galactosidase E-8900 Ethyl-beta-D-thiogalactopyranoside
I-8000 Isopropyl-beta-D- thiogalactopyranoside
M-4440 Methyl- beta-D- thiogalactopyranoside
M-3591 1-O-Methyl- beta-D- galactopyranoside
alpha-Glucosidase M-3594 3-O-Methyl-alpha-D-glucopyranoside
beta-Glucosidase M-3592 1-O-Methyl-beta-D- glucopyranoside
beta-Glucuronidase I-8350 Isopropyl-eta-D-thioglucuronic acid, sodium salt
M-3600 1-O-Methylglucuronic acid, sodium salt
alpha-Mannosidase M-4150 1-O-Methyl-alpha-D-mannopyranoside
Alkaline phosphatase N-8100 NBT chloride
T-2300 TNBT chloride
beta-Xylosidase M-6600 1-O-Methyl-beta-D-xylopyranoside
Miscellaneous I-1900 Indole-3-acrylic acid

假单胞铜绿菌中分离
C-390是一种抗菌剂加入到琼脂培养基是，可以有效地将假单胞铜绿菌从其他细菌分离出来。

C-390, 9-Chloro-9-(4’-diethyamino) phenyl-9,10-dihydro-10-phenylacridine, hydrochloride (catalog number:C-3900)
Isolation of Pseudomonas aeruginosa
C-390 is an antibacterial agent which is useful for the selective isolation of Pseudononas aeruginosa from other bacterial species when added to an agar medium.
Patent: U.S. 4,263,398(4/21/81)

使用情况：多种细菌最小抑制浓度实验的结果表示，C-390在25ug/ul或更少浓度时，抑制微生物（除假单）的生长，然而MICS包含从50到大于100ug/ml的假单胞菌铜绿菌，因此C-390被评价为是在假单胞菌琼脂F中的最有潜力的选择性选择试剂，修复试验在53株假单胞菌铜绿菌的存在下进行，结果和在其他培养基作对比，显示假单胞菌琼脂F含30ug/ml C-390抑制性小而选择性大，加入C-390的假单胞菌琼脂F，有选择性和差异性，大量的证据同样告诉我们，C-390可以被加入到其他基础培养基中，得到同样的结果，文献著作权属SEEBIO所有。

Use: Results of minimal inhibitory concentration tests with a diversity of bacterial strains showed that C-390 inhibited the growth of all microorganisms tested (other than Pseudononas aeruginosa) at 25μg/μl or less, whereas MICs obtained for Pseudononas aeruginosa ranged from 50 to >100μg/ml. Therefore, C-390 was evaluated as a potential selective agent for Pseudononas aeruginosa in pseudomonas agar F.Recovery tests were conducted on this medium with 53 strains of Pseudononas aeruginosa, and the results were compared to those obtained in similar tests on commercially available selective media, i.e. pseudomonas isolation agar and Psersosel agar. The results of these comparisons indicated that pseubomonas agar F with C-390(30μg/ml) was significantly less inhibitory than Pseudosel agar and pseudomonas isolation agar and more selective than pseudomonas isolation agar. The incorporation of C-390 in pseudomonas agar F also provided a medium that was both selective and differential. Preliminary evidence also suggested that C-390 may be added to other basal media with comparable results. Literature is available from SEEBIO.

GENERAL MICROBIOLOGY 普通微生物学：
显色酶底物被很广泛的用于食品微生物学，环境微生物学，临床微生物学
SEEBIO开发了大量的其他底物，请检查我们的酶在字母排列表中的以考信息，另外，我们鼓励您因为其他的您需要的但不在我们目录中的底物而联系我们，我们很高兴去寻找您所需要的合成的产品的可能性。

Chromogenic enzyme substrates are widely used in
FOOD MICROBIOLOGY
ENVIRONMENTAL MICROBIOLOGY
CLINICAL MICROBIOLOGY

SEEBIO carries a large variety of these and other substrates. Please check out our enzyme cross reference listing and/or our detailed alphabetical listing. Furthermore, we encourage you to contact SEEBIO regarding any substrate that you may need, but we do not list in our catalog. We will be glad to explore the possibilities for custom synthesis of your substrate of choice.

CHROMOGENIC SUBSTRATES
Indoxyl Substrates
e.g. 5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside (X-gal) Catalog-#B-7150
The same basic reaction applies to the following chromogenic indigo substrate derivatives:
Substrates Color of precipitate Absorption λmax[nm]
X Blue 615
YTM Blue 680
MagentaTM Magenta 565
SalmonTM Salmon 540
Iodo Purple 575
GreenTM green 665

SEEBIO’s product:
BLUE I:
Substrates for Glycosidases:
B-6950 X-β-D-galactosaminide 5-Bromo-4-chloro-3-indoxyl-N-acetyl-beta-D-galactosaminide
B-7000 X-β-D-glucosaminide 5-Bromo-4-chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide
B-7110 X-β-D-cellobioside 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside
B-7135 X-α-L-fucoside 5-Bromo-4-chloro-3-indoxyl-alpha-L-fucopyranoside
B-7136 X-β-D-fucoside 5-Bromo-4-chloro-3-indoxyl-beta-D- fucopyranoside
B-7138 X-β-L-fucoside 5-Bromo-4-chloro-3-indoxyl-beta-L- fucopyranoside
B-7141 X-α-D-gal 5-Bromo-4-chloro-3-indoxyl-alpha-D-galactopyranoside
B-7150 X-gal, X-β-D-gal 5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside
B-7230 X-α-D-glc 5-Bromo-4-chloro-3-indoxyl-alpha-D-glucopyranoside
B-7250 X-β-D-glc 5-Bromo-4-chloro-3-indoxyl-beta-D-glucopyranoside
B-7300 X-β-D-glcUA·CHX 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7400 X-β-D-glcUA·Na 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt
B-7407 X-α-D-maltotrioside 5-Bromo-4-chloro-3-indoxyl-alpha-D-maltotrioside
B-7409 X-α-D-mannoside 5-Bromo-4-chloro-3-indoxyl-alpha-D-mannopyranoside
B-7750 X-β-D-xyl 5-Bromo-4-chloro-3-indoxyl-beta-D-xylopyranoside

Substrates for Esterases:
B-7050 X-butyrate 5-Bromo-4-chloro-3-indoxyl butyrate
B-7100 X-caprylate 5-Bromo-4-chloro-3-indoxyl caprylate
B-7410 X-nonanoate 5-Bromo-4-chloro-3-indoxyl nonanoate
B-7412 X-oleate 5-Bromo-4-chloro-3-indoxyl oleate
B-7414 X-palmitate 5-Bromo-4-chloro-3-indoxyl palmitate

Substrate for Phosphatases:
B-7420 X-phos·diAMPD 5-Bromo-4-chloro-3-indoxyl phosphate, di(2-amino-2-methyl-1,3-propanedio) salt
B-7450 X-phos·diNa 5-Bromo-4-chloro-3-indoxyl phosphate, disodium salt
B-7500 X-phos·p-tol 5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt

Substrate for Sulfatases:
B-7600 X-sulfate·K 5-Bromo-4-chloro-3-indoxyl sulfate, potassium salt

BLUE II:
Substrates for Glycosidases:
I-6000 YTM -β-D- gal 3-Indoxyl- beta-D- galactopyranoside
I-6050 YTM -β-D- glc 3-Indoxyl- beta-D- glucopyranoside
I-6100 YTM -β-D-glcUA·CHX 3-Indoxyl- beta-D- glucuronic acid, cyclohexlammonium salt
I-6140 YTM -β-D-glcUA·Na 3-Indoxyl- beta-D- glucuronic acid, sodium salt

Substrate for Esterases:
I-5680 YTM -butyrate 3-Indoxyl butyrate

Substrates for Phosphatases:
I-6200 YTM - phos·diAMPD 3-Indoxyl- phosphate, di(2-amino-2-methyl-1,3-propanedio) salt
I-6250 YTM - phos·diNa 3-Indoxyl- phosphate, disodium salt
I-6300 YTM - phos·p-tol 3-Indoxyl- phosphate, p-toluidine salt

Substrate for Sulfatases:
I-6350 YTM - sulfate·K 3-Indoxyl- sulfate, potassium salt

MAGENTA:
Substrates for Glycosidases:
B-7020 MagentaTM -β-D-glucosaminide 5-Bromo-6-chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide
B-7195 MagentaTM -α-D-gal 5-Bromo-6-chloro-3-indoxyl-alpha-D-galactopyranoside
B-7200 MagentaTM -β-D-gal 5-Bromo-6-chloro-3-indoxyl-beta-D- galactopyranoside
B-7270 MagentaTM -β-D-glc 5-Bromo-6-chloro-3-indoxyl- beta-D-glucopyranoside
B-7350 MagentaTM -β-D-glcUA 5-Bromo-6-chloro-3-indoxyl-beta-D- glucuronic acid, cyclohexylammonium salt

Substrates for Esterases:
B-7060 MagentaTM -butyrate 5-Bromo-6-chloro-3-indoxyl-butyrate
B-7095 MagentaTM -caprate 5-Bromo-6-chloro-3-indoxyl-caprate
B-7102 MagentaTM -caprylate 5-Bromo-6-chloro-3-indoxyl-caprylate
B-7411 MagentaTM -nonaoate 5-Bromo-6-chloro-3-indoxyl- nonaoate
B-7416 MagentaTM -palmitate 5-Bromo-6-chloro-3-indoxyl-palmitate

Substrate for Phosphatases:
B-7550 MagentaTM - phos·p-tol 5-Bromo-6-chloro-3-indoxyl phosphate, p-toluidine salt

Substrate for Sulfatases
B-7650 MagentaTM - sulfate·K 5-Bromo-6-chloro-3-indoxyl phosphate, potassium salt

SALMON:
Substrates for Glycosidases:
C-4762 SalmonTM -β-D-galactosaminide 6-chloro-3-indoxyl- N-acetyl-beta-D-galactosaminide
C-4830 SalmonTM -β-D-cellobioside 6-chloro-3-indoxyl- beta-D-cellobioside
C-4995 SalmonTM -α-D-gal 6-chloro-3-indoxyl- alpha-D-galactopyranoside
C-5000 SalmonTM -β-D-gal 6-chloro-3-indoxyl- beta-D- galactopyranoside
C-5020 SalmonTM -β-D-glc 6-chloro-3-indoxyl- beta-D-glucopyranoside
C-5050 SalmonTM -β-D-glcUA·CHX 6-chloro-3-indoxyl- beta-D-glucuronic acid, cyclohexylammonium salt
C-5070 SalmonTM -α-D-mannoside 6-chloro-3-indoxyl- alpha-D-mannopyranoside

Substrates for Esterases:
C-4800 SalmonTM -butyrate 6-chloro-3-indoxyl butyrate
C-4820 SalmonTM -caprylate 6-chloro-3-indoxyl caprylate
C-5075 SalmonTM -nonanoate 6-chloro-3-indoxyl nonanoate
C-5080 SalmonTM -palmitate 6-chloro-3-indoxyl palmitate

Substrates for Phosphatases:
C-5100 SalmonTM -phos·p-tol 6-chloro-3-indoxyl phosphate, p-toluidine salt

PURPLE:
I-7600 Iodo -β-D-gal 5-Iodo-3-indoxyl-beta-D-galactopyranoside

GREEN:
M-4100 GreenTM -β-D-gal N-Methylindoxyl-beta-D-galactopyranoside

Oxidizing Agents:
N-8100 NBT Nitrotetrazolium blue chloride
T-2300 TNBT Tetranitro blue tetrazolium chloride

2-and 4-Nitrophenyl (ONP and PNP) Substrates
e.g. 4-Nitrophenyl phosphate, disodium salt (PNP-phos) Catalog-#:N-5010
The same basic reaction applies for all 2-and 4-Nitrophenyl (ONP and PNP) substrates.

SEEBIO’s products:
Substrates for Glycosidases:
N-3970 PNP-α-D-galactosaminide 2-Nitrophenyl-N-acetyl-alpha-D-galactosaminide
N-3990 PNP-β-D-galactosaminide 4-Nitrophenyl-N-acetyl-beta-D-galactosaminide
N-4014 ONP-α-D-glucosaminide 2-Nitrophenyl-N-acetyl-alpha-D-glucosaminide
N-4022 ONP-β-D-glucosaminide 2-Nitrophenyl-N-acetyl-beta-D-glucosaminide
N-4030 PNP-α-D- glucosaminide 4-Nitrophenyl-N-acetyl-alpha-D- glucosaminide
N-4050 PNP-β-D- glucosaminide 4-Nitrophenyl-N-acetyl- beta -D- glucosaminide
N-4070 PNP-α-L-arabinofuranoside 4-Nitrophenyl-alpha-L-arabinofuranoside
N-4100 PNP-β-D-cellobiose 4-Nitrophenyl – beta-D-cellobioside
N-4130 PNP-α-L-fucoside 4-Nitrophenyl-alpha-L-fucopyranoside
N-4150 PNP-β-D-fucoside 4-Nitrophenyl- beta-D -fucopyranoside
N-4160 PNP-β-L-fucoside 4-Nitrophenyl- beta-L -fucopyranoside
N-4190 ONP-β-D-gal 2-Nitrophenyl-beta-D-galactopyranoside
N-4200 PNP-α- D-gal 4-Nitrophenyl- alpha -D-galactopyranoside
N-4300 PNP-β- D-gal 4- Nitrophenyl-beta -D-galactopyranoside
N-4320 ONP-β-D-glc 2- Nitrophenyl-beta -D-glucopyranoside
N-4330 MNP-β-D-glc 3- Nitrophenyl-beta -D-glucopyranoside
N-4350 PNP-α- D-glc 4-Nitrophenyl- alpha -D- glucopyranoside
N-4400 PNP-β- D-glc 4-Nitrophenyl- beta -D- glucopyranoside
N-4450 PNP-β- D-glcUA 4-Nitrophenyl- beta -D- glucuronic acid
N-4465 PNP-β- D-glcUA·Na 4-Nitrophenyl- beta -D- glucuronic acid, sodium salt
N-4480 PNP-β- D-lac 4-Nitrophenyl- beta -D-lactopyranoside
N-4487 PNP-α- D-maltopentaoside 4-Nitrophenyl-alpha-D-maltopentaoside
N-4490 PNP-α- D-maltoside 4-Nitrophenyl-alpha-D-maltopyranoside
N-4492 PNP-β- D- maltoside 4-Nitrophenyl- beta -D-maltopyranoside
N-4500 PNP-α- D-mannoside 4-Nitrophenyl-alpha-D-mannopyranoside
N-4550 PNP-α- D-mannoside 4-Nitrophenyl-beta-D-mannopyranoside
N-5290 ONP-β- D-xyl 2-Nitrophenyl-beta-D-xylopyranoside
N-5300 PNP-β- D-xyl 4-Nitrophenyl-beta-D-xylopyranoside

Substrate for Esterases:
N-4600 PNP-palmitate 4-Nitrophenyl palmitate

Substrates for Phosphatases:
N-4700 PNP-phos·CHX 4-Nitrophenyl phosphate, dicyclohexylammonium salt
N-5000 PNP-phos·diNa 4-Nitrophenyl phosphate, disodium salt
Horseradish Peroxidase/TMB System

SEEBIO’s products:
T-2100 TMB 3,3’,5,5’-Tetramethylbenzidine, free base
T-2150 TMB·diHCl 3,3’,5,5’-Tetramethylbenzidine dihydrochloride

十三. FLUOROGENIC SUBSTRATES
Methylumbelliferyl (4-MU-) Substrates
e.g. 4-Methylumbelliferyl-beta-D-glucuronic acid (4-MU-glcUA) Catalog-#: M-5700
The same basic reaction applies for all 4-Methylumbelliferyl substrates:

SEEBIO’s products:
Substrates for Glycosidases:
M-5500 4-MU-β- D- galactosaminide 4-Methylumbelliferyl-N-acetyl-beta-D-galactosaminide
M-5504 4-MU-β- D- glucosaminide 4-Methylumbelliferyl-N-acetyl-beta-D-glucosaminide
M-5510 4-MU-α- L- arabinoside 4-Methylumbelliferyl-alpha-L-arabinopyranoside
M-5530 4-MU-β- D-cellobioside 4-Methylumbelliferyl- beta-D-cellobioside
M-5539 4-MU-α- L-fucoside 4-Methylumbelliferyl-alpha-L-fucopyranoside
M-5540 4-MU-β- D-fucoside 4-Methylumbelliferyl-beta-D-fucopyranoside
M-5541 4-MU-β- L-fucoside 4-Methylumbelliferyl-beta-L-fucopyranoside
M-5544 4-MU-α-D-gal 4-Methylumbelliferyl-alpha-D-galactopyranoside
M-5550 4-MU-β-D-gal 4-Methylumbelliferyl-beta-D-galactopyranoside
M-5600 4-MU-α-D-glc 4-Methylumbelliferyl-alpha-D-glucopyranoside
M-5650 4-MU-β-D-glc 4-Methylumbelliferyl-beta-D-glucopyranoside
M-5700 MUG, 4-MU-β-D-glcUA 4-Methylumbelliferyl-beta-D-glucuronic acid
M-5718 4-MU-β-D-lactoside 4-Methylumbelliferyl-beta-D-lactoside
M-5730 4-MU-α-D-mannoside 4-Methylumbelliferyl-alpha-D-mannopyranoside
M-5738 4-MU-β-D-mannoside 4-Methylumbelliferyl-beta-D-mannopyranoside
M-6500 4-MU-β-D-xyl 4-Methylumbelliferyl-beta-D-xylopyranoside

Substrates for Esterases:
M-5520 4-MU-butyrate 4-Methylumbelliferyl butyrate
M-5526 4-MU-caprylate 4-Methylumbelliferyl caprylatd
M-5744 4-MU-nonanoate 4-Methylumbelliferyl nonanoate
M-5750 4-MU-palmitate 4-Methylumbelliferyl palmitate

Substrates for Phosphatases:
B-3500 2(4-MU)-phos Bis(4-methylumbelliferyl) phosphate
M-5800 4-MU-phos 4-Methylumbelliferyl phosphate, free acid
M-5850 4-MU-phos·diAMPD 4-Methylumbelliferyl phosphate, di(2-amino-2-methyl-1,3-propanedio) salt
M-5900 4-MU-phos·diCHX 4-Methylumbelliferyl phosphate, dicyclohexylammonium
M-5920 4-MU-phos·diLi 4-Methylumbelliferyl phosphate, dilithium salt
M-5950 4-MU-phos·diNa 4-Methylumbelliferyl phosphate, disodium salt trihydrate

Substrate for Sulfatases:
M-6200 4-MU-sulfate·K 4-Methylumbelliferylsulfate, potassium salt

十四. 7-Amido-4-methylcoumarin (AMC) Substrate
e.g. Lysine 7-Amido-4-methylcoumarin Catalog-#: L-9300
The same basic reaction applies for all 7-Amido-4-methylcoumarin (AMC) substrates.

SEEBIO’s products:
A-1470 Ac-Lys-AMC·AcOH N-Acetyl-L-lysine 7-amido-4-methylcoumarin, acetate salt
A-4220 H-D-Ala- AMC·TFA D-Alanine 7-amido-4-methylcoumarin, trifluoroacetate salt
A-4500 H-Ala- AMC·TFA L- Alanine 7-amido-4-methylcoumarin, trifluoroacetate salt
A-8700 H-Arg- AMC·diHCl L-Arginine 7-amido-4-methylcoumarin dihydrochloride
A-8750 H- Arg-Arg- AMC·HCl L-Arginyl-L-arginine 7-amido-4-methylcoumarin hydrochloride
A-9300 H-Asp-AMC L-Aspartic acid alpha- (7-amido-4-methylcoumarin)
A-9305 H-Asp-AMC·TFA L-Aspartic aicd alpha- (7-amido-4-methylcoumarin), rtifluoroacetate salt
C-2100 H-α-CBZ-Arg- AMC·HCl N-alpha-CBZ-L-Arginie (7-amido-4-methylcoumarin) hydrochloride
C-2128 CBZ-Lys-Lys-Arg- AMC·triAcOH N-CBZ-Lysyl-lysyl-arginine 7-amido-4-methylcoumarin, triacetate salt
C-2150 N-CBZ-Gly-Pro-AMC N-CBZ-Glycyl-L-proline 7-amido-4-methylcoumarin
C-6200 H-Cit- AMC·HBr L-Citrulline 7-amido-4-methylcoumarin hydrobromide
C-6300 H- Cit- AMC·TFA L-Citrulline 7-amido-4-methylcoumarin, trifluoroacetate salt
G-3845 H-Glu(OH)-AMC L-Glutamic acid alpha-(7-amido-4-methylcoumarin)
G-3850 H-Glu-AMC L-Glutamic acid gamma-(7-amido-4-methylcoumarin)
G-3950 Glutaryl-Gly-Arg-AMC Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin
G-4500 H-Gly- AMC·HBr Glycine 7-amido-4-methylcoumarin hydrobromide
G-6100 H-Gly-Gly-AMC·HCl Glycyl-glycine 7-amido-4-methylcoumarin hydrochloride
G-6300 Gly-Phe-AMC Glycyl-L-phenylalanine 7-amido-4-methylcoumarin
G-6350 Gly-Pro- AMC·HBr Glycyl-L-proline 7-amido-4-methylcoumarin hydrobromide
H-2350 H-His-AMC L-Histidine 7-amido-4-methylcoumarin
H-7300 H-Hyp-AMC L-Hydroxyproline 7-amido-4-methylcoumarin
I-7900 H-lle- AMC·TFA L-Isoleucine 7-amido-4-methylcoumarin, trifluoroacetate salt
L-2750 H-Leu-AMC L-Leucine 7-amido-4-methylcoumarin
L-2800 H-Leu-AMC·HCl L-Leucine 7-amido-4-methylcoumarin hydrochloride
L-9300 H-Lys- AMC·AcOH L-Lysine 7-amido-4-methylcoumarin, acetate salt
M-3170 H-Met- AMC·AcOH L-Methionine 7-amido-4-methylcoumarin, acetate salt
M-3200 H- Met- AMC·TFA L-Methionine 7-amido-4-methylcoumarin, trifluoroacetate salt
O-3400 H-Orn-AMC·Cab L-Ornithine 7-amido-4-methylcoumarin, carbonate salt
P-3500 H-Phe- AMC·TFA L-Phenylalanine 7-amido-4-methylcoumarin, trifluoroacetate salt
P-7280 H-Pro- AMC·HBr L-Proline 7-amido-4-methylcoumarin hydrobromide
P-8500 H-Pyr-AMC L-Pyroglutamic acid 7-amido-4-methylcoumarin
S-2700 H-Ser- AMC·HCl L-Serine 7-amido-4-methylcoumarin hydrochloride
S-3300 H-Ser-Tyr-AMC L-Seryl-L-tyrosine 7-amido-4-methylcoumarin
T-8350 H-Trp-AMC L-Tryptophan 7-amido-4-methylcoumarin
T-8355 H-Trp- AMC·HCl L- Tryptophan 7-amido-4-methylcoumarin hydrochloride
T-9100 H-Tyr-AMC L-Tyrosine 7-amido-4-methylcoumarin
V-2000 H-Val-AMC L-Valine 7-amido-4-methylcoumarin
V-2100 H-Val- AMC·TFA L- Valine 7-amido-4-methylcoumarin, trifluoroacetate salt
Different salts and N-protected AMC derivatives for peptide synthesis are also available from BIOSYNH.
Please inquire for any AMC substrate that may be needed in your application.

BIOLUMINESCENT SUBSTRATES
Firefly Luciferase Substrates
e.g. Luciferin Firefly, free acid, synth. Catalog-#: L-8200
The same basic reaction occurs when Luciferase is added to the system or Luciferase is expressed by a reporter gene.

SEEBIO’s products:
L-8200 D-Luciferin Firefly (synth.)
L-8220 D-Luciferin Firefly, potassium salt (synth.)
L-8240 D- Luciferin Firefly, sodium slat (synth.)
L-8080 Luciferase, American Firetly, natural

Apoaequorin-and Renilla Luciferase-Substrate: Coelenterazine catalog-#: C-7000
The different wavelengths compared to applications with Firefly Luciferase make double reporter gene applications possible.

SEEBIO’s products:
C-7000 Coelenterazine
D-0400 2-(4-Dehydroxy) coelenterazine

Glycosidases:
alpha-Amylase B-7407 5-Bromo-4-Chloro-3-indoxyl-alpha-D-maltotrioside
N-4487 4-Nitrophenyl-alpha-D-maltopentaoside
alpha-L-Arabinosidase M-5510 4-Methylumbelliferyl-alpha-L-arabinopyranoside
N-4070 4-Nitrophenyl-alpha-L-arabinofuranoside
beta-Cellobiosidase B-7110 5- Bromo-4-Chloro-3-indoxyl-beta-D-cellobioside
C-4830 6-Chloro-3-indoxyl-beta-D-cellobioside
M-5530 4-Methylumbelliferyl-beta-D- cellobioside
N-4100 4-Nitrophenyl- beta-D- cellobioside
alpha-L-Fucosidase B-7135 5-Bromo-4-Chloro-3-indoxyl-alpha-L-fucopyranoside
N-2230 2-Naphthyl-alpha-L- fucopyranoside
M-5539 4-Methylumbelliferyl- alpha-L- fucopyranoside
N-4130 4-Nitrophenyl- alpha-L- fucopyranoside
beta-D-Fucosidase B-7136 5-Bromo-4-Chloro-3-indoxyl-beta-D-fucopyranoside
M-5540 4-Methylumbelliferyl-beta-D- fucopyranoside
N-4150 4-Nitrophenyl-beta-D- fucopyranoside
beta-L-Fucosidase B-7138 5-Bromo-4-Chloro-3-indoxyl-beta-L-fucopyranoside
M-5541 4-Methylumbelliferyl-beta-L- fucopyranoside
N-4160 4-Nitrophenyl-beta-L- fucopyranoside
alpha-Galactosaminidase N-3970 2-Nitrophenyl-N-acetyl-alpha-D-galactosaminide
beta- Galactosaminidase B-6950 5-Bromo-4-Chloro-3-indoxyl-N-acetyl-beta-D-galactosaminide
C-4762 6-Chloro-3-indoxyl-N-acetyl-beta-D-galactosaminide
M-5500 4-Methylumbelliferyl-N-acetyl-beta-D-galactosaminide
N-3990 4-Nitrophenyl-N-acetyl-beta- D-galactosaminide
alpha-Galactosidase B-7141 5-Bromo-4-Chloro-3-indoxyl-alpha-D-galactopyranoside
B-7195 5-Bromo-4-Chloro-3-indoxyl-alpha-D-galactopyranoside
C-4995 6-Chloro-3-indoxyl-alpha-D-galactopyranoside
M-5544 4-Methylumbelliferyl-alpha-D-galactopyranoside
N-2250 1-Naphthyl-alpha-D-galactopyranoside
N-4200 4-Nitrophenyl-alpha-D-galactopyranoside
beta- Galactosidase A-7050 4-Aminophenyl-beta-D- galactopyranoside
B-7150 5-Bromo-4-Chloro-3-indoxyl-beta-D-galactopyranoside
B-7200 5-Bromo-6-Chloro-3-indoxyl-beta-D-galactopyranoside
B-8900 5-Bromo-3-indoxyl-beta-D-galactopyranoside
C-5000 6-Chloro-3-indoxyl-beta-D-galactopyranoside
F-5500 6-Fluoro-3-indoxyl-beta-D-galactopyranoside
H-7500 8-Hydroxyquinoline- beta-D-galactopyranoside
I-6000 3-Indoxyl-beta-D-galactopyranoside
I-7600 5-Iodo-3-indoxyl-beta-D-galactopyranoside
M-4100 N-Methylindoxyl-beta-D-galactopyranoside
M-5550 4-Methylumbelliferyl-beta-D-galactopyranoside
N-2030 Naphthol AS-BI-beta-D-galactopyranoside
N-2260 2-Naphthyl- beta-D-galactopyranoside
N-4190 2-Nitrophenyl-beta-D-galactopyranoside
N-4300 4-Nitrophenyl-beta-D-galactopyranoside
P-4100 Phenyl-beta-D-galactopyranoside
alpha-Glucosaminidase N-4014 2-Nitrophenyl-N-acetyl-alpha-D-glucosaminide
N-4030 4-Nitrophenyl-N-acetyl-alpha-D-glucosaminide
beta-Glucosaminidase B-7000 5-Bromo-4-Chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide
B-7020 5-Bromo-6-Chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide
M-5504 4-Methylumbelliferyl-N-acetyl-beta-D-glucosaminide
N-4022 2-Nitrophenyl-N-acetyl-beta-D-glucosaminide
N-4050 4-Nitrophenyl-N-acetyl-beta-D-glucosaminide
alpha-Glucosidase B-7230 5-Bromo-4-Chloro-3-indoxyl-alpha-D-glucopyranoside
M-5600 4-Methylumbelliferyl-alpha-D-glucopyranoside
N-2300 2-Naphthyl-alpha-D-glucopyranoside
N-4350 4-Nitrophenyl-alpha-D-glucopyranoside
beta-Glucosidase B-7250 5-Bromo-4-Chloro-3-indoxyl-beta-D-glucopyranoside
B-7270 5-Bromo-6-Chloro-3-indoxyl-beta-D-glucopyranoside
C-5020 6-Chloro-3-indoxyl-beta-D-glucopyranoside
I-6050 3-Indoxyl-beta-D-glucopyranoside
M-5650 4-Methylumbelliferyl-beta-D-glucopyranoside
N-2210 1-Naphthyl-N-acetyl-glucosaminide
N-2290 1-Naphthyl-beta-D-glucopyranoside
N-4320 2-Nitrophenyl-beta-D-glucopyranoside
N-4330 3-Nitrophenyl-beta-D-glucopyranoside
N-4400 4-Nitrophenyl-beta-D-glucopyranoside
P-4100 Phenyl-beta-D-galactopyranoside
P-4130 Phenyl-beta-D-glucopyranoside
beta-Glucuronidase B-7300 5-Bromo-4-Chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylamonium salt
B-7350 5-Bromo-6-Chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7400 5-Bromo-4-Chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt
B-8902 5-Bromo-4-Chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
C-5050 6-Chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
H-7600 8-Hydroxyquinoline-beta-D-glucuronic acid
I-6100 3-Indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
I-6140 3-Indoxyl-beta-D-glucuronic acid, sodium salt
M-5700 4-Methylumbelliferyl-beta-D-glucuronic acid
N-4450 4-Nitrophenyl-beta-D-glucuronic acid
N-4465 4-Nitrophenyl-beta-D-glucuronic acid, sodium salt
P-4150 Phenyl-beta-D-glucuronic acid
beta-Lactosidase M-5718 4-Methylumbelliferyl-beta-D-lactoside
N-4480 4-Nitrophenyl-beta-D-lactopyranoside
alpha-Maltosidase N-4490 4-Nitrophenyl-alpha-D-maltopyranoside
alpha-Mannosidase B-7409 5-Bromo-4-Chloro-3-indoxyl-alpha-D-mannopyranoside
B-8930 2-(6-Bromonaphthyl)-alpha-D-mannopyranoside
C-5070 6-Chloro-3-indoxyl-alpha-D-mannopyranoside
M-5730 4-Methylumbelliferyl-alpha-D-mannopyranoside
N-4500 4-Nitrophenyl-alpha-D-mannopyranoside
beta-Mannosidase M-5738 4-Methylumbelliferyl-beta-D-mannopyranoside
N-4550 4-Nitrophenyl-beta-D-mannopyranoside
beta-Xylosidase B-7750 5-Bromo-4-Chloro-3-indoxyl-beta-D-xylopyranoside
B-8940 2-(6-Bromonaphthyl)-beta-D-xylopyranoside
M-6500 4-Methylumbelliferyl-beta-D-xylopyranoside
N-5290 2-Nitrophenyl-beta-D-xylopyranoside
N-5300 4-Nitrophenyl-beta-D-xylopyranoside

Esterases:
Carboxyl Esterase B-7050 5-Bromo-4-Chloro-3-indoxyl butyrate
B-7100 5-Bromo-4-Chloro-3-indoxyl caprylate
Cholinesterase B-9600 S-Butyrythiocholine iodide

Esterase(misc.)
B-6244 5-Bromo-4-Chloro-3-indoxyl-3-acetate
B-6304 5-Bromo-6-Chloro-3-indoxyl-3-acetate
B-7050 5-Bromo-4-Chloro-3-indoxyl butyrate
B-7060 5-Bromo-6-Chloro-3-indoxyl butyrate
B-7095 5-Bromo-6-Chloro-3-indoxyl caprate
B-7100 5-Bromo-4-Chloro-3-indoxyl caprylate
B-7102 5-Bromo-6-Chloro-3-indoxyl caprylate
B-7140 5-Bromo-4-Chloro-3-indoxyl nonanoate
B-7411 5-Bromo-6-Chloro-3-indoxyl nonanoate
B-7412 5-Bromo-4-Chloro-3-indoxyl oleate
B-7414 5-Bromo-4-Chloro-3-indoxyl palmitate
B-7416 5-Bromo-6-Chloro-3-indoxyl palmitate
C-4758 6-Chloro-3-indoxyl-3-acetate
C-4800 6-Chloro-3-indoxyl butyrate
C-4820 6-Chloro-3-indoxyl caprylate
C-5075 6-Chloro-3-indoxyl nonanoate
C5080 6-Chloro-3-indoxyl palmitate
I-5600 3-Indoxyl-3-acetate
I-5680 3-Indoxyl butyrate
M-5520 4-Methylumbelliferyl butyrate
M-5526 4-Methylumbelliferyl caprylate
M-5744 4-Methylumbelliferyl nonanoate
M-5750 4-Methylumbelliferyl palmitate
N-4600 4-Nitrophenyl palmitate

Phosphatases:
Alkaline Phosphatase B-7450 5-Bromo-4-Chloro-3-indoxyl phosphate, disodium salt
B-7500 5-Bromo-4-Chloro-3-indoxyl phosphate, p-toluidine salt
B-7550 5-Bromo-6-Chloro-3-indoxyl phosphate, p-toluidine salt
C-5100 6-Chloro-3-indoxyl phosphate, p-toluidine salt
I-6200 3-Indoxyl phosphate, di(2-amino-2-methyl-1,3-propanedio) salt
I-6250 3-Indoxyl phosphate, disodium salt
I-6300 3-Indoxyl phosphate, p-toluidine salt
M-5800 4-Methylumbelliferyl phosphate, free acid
M-5850 4-Methylumbelliferyl phosphate, di(2-amino-2-methyl-1,3-propanedio) salt
M-5900 4-Methylumbelliferyl phosphate, dicyclohexylammonium salt
M-5920 4-Methylumbelliferyl phosphate, dilithium salt
M-5950 4-Methylumbelliferyl phosphate, disodium salt trihydrate

Phosphatase(misc.)
B-3500 Bis (4-methylumbelliferyl) phosphate
B-7420 5-Bromo-4-Chloro-3-indoxyl phosphate, di(2-amino-2-methyl-1,3-propanedio) salt
N-2370 1-Naphthyl phosphate, calcium salt
N-2400 1-Naphthyl phosphate, sodium salt
N-2450 2-Naphthyl phosphate, monosodium salt
N-4700 4-Nitrophenyl phosphate, dicyclohexylammonium salt
N-5000 4-Nitrophenyl phosphate, disodium salt
P-2360 Phenolphthalein diphosphate, tetrasodium salt

Peptidases:
Cathepsin A-8700 L-Arginine 7-amido-4-methylcoumarin dihydrochloride
Dipeptidyl Aminopeptidase II L-9400 L-Lysyl-L-alanine-4-methoxy-beta-naphthylamide hydrobromide
Gamma-Glutamyl Transpeptidase G-3850 L-Glutamic acid gamma-(7-amido-4-methylcoumarin)
G-3920 L-gamma-Glutamyl-4-nitroanilide monohydrate
Pyroglutamyl Peptidase P-8500 L-Pyroglutamic acid 7-amido-4-methylcoumarin
P-8600 L-Pyroglutamic acid-2-naphthylamide

Peptidase(misc.)
A-1470 N-Acetyl-L-lysine 7-amido-4-methylcoumarin, acetate salt
A-4220 D-Alanine 7-amido-4-methylcoumarin, trifluoroacetate salt
A-4500 L-Alanine 7-amido-4-methylcoumarin, trifluoroacetate salt
A-8700 L-Arginine 7-amido-4-methylcoumarin hydrochloride
A-8750 L-Arginyl-L-arginine 7-amido-4-methylcoumarin dihydrochloride
A-9300 L-Aspartic acid alpha-(7-amido-4-methylcoumarin)
A-9305 L-Aspartic acid alpha-(7-amido-4-methylcoumarin), trifluoroacetate salt
C-2100 N-alpha-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride
C-2128 N-CBZ-Lysyl-lysyl-arginine 7-amido-4-methylcoumarin, triacetate salt
C-2150 N-CBZ-Glycyl-L-proline 7-amido-4-methylcoumarin
C-6200 L-Citrulline 7-amido-4-methylcoumarin hydrobromide
C-6300 L-Citrulline 7-amido-4-methylcoumarin, trifluoroacetate salt
G-3845 L-Glutamic acid alpha-(7-amido-4-methylcoumarin)
G-3850 L-Glutamic acid gamma-(7-amido-4-methylcoumarin)
G-3950 Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride
G-4500 Glycine 7-amido-4-methylcoumarin hydrobromide
G-6100 Glycyl-glycine 7-amido-4-methylcoumarin hydrochloride
G-6300 Glycyl-L-phenylalanine 7-amido-4-methylcoumarin
G-6350 Glycyl-L-proline 7-amido-4-methylcoumarin hydrobromide
H-2350 L-Histidine 7-amido-4-methylcoumarin
H-7300 L-Hydroxyproline 7-amido-4-methylcoumarin hydrochloride
I-7900 L-Isoleucine 7-amido-4-methylcoumarin, trifluoroacetate salt
L-2750 L-Leucine 7-amido-4-methylcoumarin
L-2800 L-Leucine 7-amido-4-methylcoumarin hydrochloride
L-9300 L-Lysine 7-amido-4-methylcoumarin, acetate salt
M-3170 L-Methionine 7-amido-4-methylcoumarin, acetate salt
M-3200 L-Methionine 7-amido-4-methylcoumarin, trifluoroacetate salt
O-3400 L-Ornithine 7-amido-4-methylcoumarin, carbonate salt
P-3500 L-Phenylalanine 7-amido-4-methylcoumarin, trifluoroacetate salt
P-7280 L-Proline 7-amido-4-methylcoumarin hydrobromide
P-8500 L-Pyroglutamic acid 7-amido-4-methylcoumarin
S-2700 L-Serine 7-amido-4-methylcoumarin hydrochloride
S-3300 L-Seryl-L-tyrosine 7-amido-4-methylcoumarin
T-8350 L-Tryptophan 7-amido-4-methylcoumarin
T-8355 L-Tryptophan 7-amido-4-methylcoumarin hydrochloride
T-9100 L-Tyrosine 7-amido-4-methylcoumarin
V-2000 L-Valine 7-amido-4-methylcoumarin
V-2100 L-Valine 7-amido-4-methylcoumarin trifluoroacetate salt

Sulfatases:
Arylsulfatase B-7600 5-Bromo-4-Chloro-3-indoxyl sulfate, potassium salt
B-7650 5-Bromo-6-Chloro-3-indoxyl sulfate, potassium salt
I-6350 3-Indoxyl sulfate, potassium salt
M-6200 4-Methylumbelliferyl sulfate, potassium salt
Sulfatases(misc.) P-2400 Phenolphthalein disulfate, tripotassium salt

Miscellaneous Enzymes:
N-AcetylneuraminicAcid Aldotase A-1600 N-Acetylneuraminic acid
Horseradish Peroxidase T-2100 3,3’,5,5’-Tetramethylbenzidine, free base
T-2150 3,3’,5,5’-Tetramethylbenzidine dihydrochloride
Luciferase L-8200 D-Luciferin Firely (synth.)
L-8220 D-Luciferin Firely, potassium salt (synth.)
L-8240 D-Luciferin Firely, sodium salt (synth.)
Phosphatidylinositol Phospholipase C(PI-PLC) B-7404 5-Bromo-4-Chloro-3-indoxyl-myo-inositol-1-phosphate, ammonium salt
N-4474 4-Nitrophenyl-myo-inositol-1-yl phosphate, ammonium salt
Phosphodiesterase I B-3700 Bis (4-nitrophenyl) phosphate, sodium salt
Phosphodiesterase II B-7680 5-Bromo-4-Chloro-3-indoxyl thymidine-3’-phosphate, cyclohexylammonium salt
Urokinase G-3950 Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride

Enzyme Inducers & Enhancers:
alpha-Galactosidase M-3590 1-O-Methyl-alpha-D-galactopyranoside
beta-Galactosidase E-8900 Ethyl-beta-D-thiogalactopyranoside
M-3591 1-O-Methyl-beta-D-galactopyranoside
M-4440 Methyl-beta-D-thiogalactopyranoside
I-8000 Isopropyl-beta-D-thiogalactopyranoside
alpha-Glucosidase M-3594 3-O-Methyl-alpha-D-glucopyranose
beta-Glucosidase I-8200 Isopropyl-beta-D-thioglucopyranoside
M-3592 1-O-Methyl-beta-D-glucopyranoside
M-4450 Methyl-beta-D-thioglucopyranoside
beta-Glucuronidase I-8350 Isopropyl-beta-D-thioglucuronic aicd, sodium salt
M-3600 1-O-Methyl-beta-D-glucuronic acid, sodium salt

alpha-Mannosidase M-4150 1-O-Methyl-alpha-D-mannopyranoside

Alkaline phosphatase
N-8100 Nitrotetrazolium blue chloride
T-2300 Tetranitro blue tetrazolium chloride
beta-Xylosidase M-6600 1-O-Methyl-beta-D-Xylopyranoside
Miscellaneous I-1900 Indole-3-acrylci acid

Cultrue Media Additives:
A-1100 N-Acetyl-L-cysteine
A-1170 N-Acetyl-D-galactosaminide
A-1200 N-Acetyl-D-glucosaminide
A-1600 N-Acetylneuraminic acid
A-3000 Adonitol
A-6100 5-Aminolevulinic acid hydrochloride
A-6150 5-Aminolevulinic aicd methyl ester hydrochloride
C-2600 D-Cellobiose
C-3900 C-390
C-5910 Cholic acid, sodium salt
D-0500 2-Deoxy-D-galactose
D-0520 2-Deoxy-D-glucose
D-0560 2-Deoxy-D-ribose
D-9500 Dulcitol
F-6300 Fluoroorotic aicd
G-1500 D-Galactosamine hydrochloride
H-1700 Hemin
M-0850 L-Malic aicd
M-1500 D-Melezitose
M-1520 D-Melezitose hydrate
M-1700 D-Melibiose monohydrate
N-2525 Neutral Red
R-1000 D-Raffinose pentahydrate
R-3000 L-Rhamnose
R-4000 Resazurin, sodium salt
R-5500 D-Ribose
S-1500 D-Salicin
S-5500 Sodium polyanetholesulfonate
S-6000 Sodium pyruvate
S-6500 Sodium taurocholate
T-3600 Thiomerosal
T-5000 D-Trehalose dihydrate
Z-3000 trans-Zeatin Riboside

Detergents
C-5910 Cholic acid, sodium salt
D-0280 n-Decyl-beta-D-glucopyranoside
D-0290 n-Decyl-4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside
D-3100 Digitonin USP24
D-3200 Digitonin special grade (water soluble)
D-8820 n-Dodecyl-beta-D-glucopyranoside
D-8823 n-Dodecyl-beta-D-maltoside
D-8824 n-Docecyl-d25-beta-D-maltoside
E-8900 Ethyl-beta-D-thiogalactopyranoside
H-1750 n-Heptyl-beta-D-glucopyranoside
H-1758 n-Heptyl-beta-D-thioglucopyranoside
H-1770 n-Hexyl-beta-D-glucopyranoside
N-8400 n-Nonyl-beta-D-glucopyranoside
O-2000 n-Octyl-beta-D-glucopyranoside
O-2700 n-Octyl-beta-D-thiogalactopyranoside
O-2710 n-Octyl-beta-D-thioglucopyranoside
S-6500 Sodium taurocholate
S-6560 Sodium taurodeoxychloate
S-6600 Sodium tauroglycocholate

Plant growth regulators
G-2700 Gibberellic acid GA3
I-1000 Indole-3-acetic acid
I-2000 Indole-3-butyric acid
K-4000 Kinetin
Z-3000 Trans-Zeatin Riboside

Oxidizing agents
N-8100 Nitrotetrazolium blue chloride
T-2300 Tetranitro blue tetrazolium chloride

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