CNP-NAG in the early diagnosis of renal injury

来源：Seebio.cn作者：西宝生物人气：-发表时间：2012-02-29 09:03:40【大中小】

CNP-NAG in the early diagnosis of renal injury

Various types of synthetic substrates have been reported for use in determinations of urinary N-acetyl-f3-D-glucosamiidase (EC 3.2.1.30; NAG) . To determine urinary NAG activity by a kinetic rate assay method, 2-chloro-4-nitrophenyl-N-acetyl-13-D-glucosaminide(CNP-NAG) is a new synthetic substrate. This method allowed good reproducibility in accordance with the reaction rate theory; required no specimen blank; was unaffected by endogenous components of urine such as glucose, ascorbic acid, and bilirubin; and could be adapted to various types of automated analyzers.

Principle
CNP-NAG, hydrolyzed by NAG , liberates the chromogen, CNP. NAG activity is determined by calculating the rate of CNP formation, in terms of the change in absorbance per minute at 400-405 nm.

Features
1)      Stability (both in powder and in solution)
2)      Operation simplification
3)      High sensitivity
4)      Good accuracy

Related product

Cat No.	Acronym	Name	CAS
DCE0085A	CNP-NAG	2-chloro-4-nitrophenyl-N-acetyl-β-D-glucosaminide	103614-82-0

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