Jackson全新推出紫外/可见光区Brilliant Violet(TM)标记二抗

来源：作者：人气：-发表时间：2017-02-28 16:56:00【大中小】

Jackson ImmunoResearch 2017年推出紫外/可见光区染料Brilliant Violet™ 421、Brilliant Violet™ 480荧光标记二抗。Brilliant Violet™ 421、Brilliant Violet™ 480与Alexa Fluor® 488、Rhodamine-Red™-X、Alexa Fluor® 647混合使用，可实现五色荧光标记体系。

Brilliant Violet 421, Brilliant Violet 488荧光标记二抗

Brilliant Violet™——更明亮的紫色激光染料

Brilliant Violet™是由Sirigen根据诺贝尔化学奖获奖成果开发的一种聚合染料，比其他紫色激光激发的染料更加明亮。Brilliant Violet™可被紫色激光器在405nm处激发，是当今市场上由紫色激发器激发的所有染料中亮度强、信噪比高的荧光材料。因此，它特别适合多色应用，用于分辨弱表达细胞群。

Brilliant Violet™适用于常用滤波器，例如采用色度技术的滤波器（如下表所示）：

Channel	Dye	Amax(nm)	Emax(nm)	Chroma Filter Set
Blue	Brilliant Violet™ 421	407	421	49027
Cyan	Brilliant Violet™ 480	436	478	49302 or 49001

Brilliant Violet™ 421、Brilliant Violet™ 480、Alexa Fluor® 488、Rhodamine-Red™-X四色标记抗体可用于核复染，用DRAQ5TMQ（红色区域发射波长，与Alexa Fluor® 647有重叠）代替常用的DAPI（蓝色区域发射波长），蓝色区域染料选用Brilliant Violet™系列（图1）。

图1 采用Brilliant Violet™421及Brilliant Violet™480双标记免疫荧光染色Hep-2细胞，
并用DRAQ5进行核复染。

Brilliant Violet™荧光标记抗体列表（订购热线：400-021-8158）

抗体描述	BV421™	BV480™
Donkey Anti-Chicken IgY (IgG)(H+L) (min X Bov, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)	0.25 ml 703-675-155	0.25 ml 703-685-155
Donkey Anti-Goat IgG (H+L) (min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)	0.25 ml 705-675-147	0.25 ml 705-685-147
Donkey Anti-Guinea Pig IgG (H+L) (min X Bov, Ck, Gt, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)	0.25 ml 706-675-148	0.25 ml 706-685-148
Donkey Anti-Human IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Ms, Rb, Rat, Shp Sr Prot)	0.25 ml 709-675-149	0.25 ml 709-685-149
Donkey Anti-Mouse IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot)	0.25 ml 715-675-150	0.25 ml 715-685-150
Donkey Anti-Mouse IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Rat, Shp Sr Prot)	0.25 ml 715-675-151	0.25 ml 715-685-151
Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rb, Sw Sr Prot)	0.25 ml 115-675-146	0.25 ml 115-685-146
Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rb, Rat Sr Prot)	0.25 ml 115-675-166	0.25 ml 115-685-166
Goat Anti-Mouse IgG Fc γ fragment specific (min X Hu, Bov, Hrs Sr Prot)	0.25 ml 115-675-071	0.25 ml 115-685-071
Goat Anti-Mouse IgM,μ chain specific (min X Hu, Bov, Hrs Sr Prot)	0.25 ml 115-675-075	0.25 ml 115-685-075
Goat Anti-Mouse IgG, Fcγ subclass 1 specific (min X Hu, Bov, Rb Sr Prot)	0.25 ml 115-675-205	0.25 ml 115-685-205
Goat Anti-Mouse IgG, Fcγ subclass 2a specific (min X Hu, Bov, Rb Sr Prot)	0.25 ml 115-675-206	0.25 ml 115-685-206
Goat Anti-Mouse IgG, Fcγ subclass 2b specific (min X Hu, Bov, Rb Sr Prot)	0.25 ml 115-675-207	0.25 ml 115-685-207
Goat Anti-Mouse IgG, Fcγ subclass 3 specific (min X Hu, Bov, Rb Sr Prot)	0.25 ml 115-675-209	0.25 ml 115-685-209
Donkey Anti-Rabbit IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp Sr Prot)	0.25 ml 711-675-152	0.25 ml 711-685-152
Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot)	0.25 ml 111-675-144	0.25 ml 111-685-144
Donkey Anti-Rat IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot)	0.25 ml 712-675-150	0.25 ml 712-685-150
Donkey Anti-Rat IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Shp Sr Prot)	0.25 ml 712-675-153	0.25 ml 712-685-153
Goat Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs, Rb Sr Prot)	0.25 ml 112-675-143	0.25 ml 112-685-143
Goat Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs, Ms, Rb Sr Prot)	0.25 ml 112-675-167	0.25 ml 112-685-167
Goat Anti-Rat IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot)	0.25 ml 112-675-071	0.25 ml 112-685-071
Goat Anti-Rat IgM,μ chain specific (min X Hu, Bov, Hrs Sr Prot)	0.25 ml 112-675-075	0.25 ml 112-685-075
Donkey Anti-Sheep IgG (H+L) (min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)	0.25 ml 713-675-147	0.25 ml 713-685-147
Controls
ChromPure Donkey IgG, whole molecule	0.25 ml 017-670-003	0.25 ml 017-680-003
ChromPure Goat IgG, whole molecule	0.25 ml 005-670-003	0.25 ml 005-680-003