LBIS(R) 小鼠卵清蛋白特异性免疫球蛋白E（OVA-IgE）ELISA试剂盒

来源：作者：人气：-发表时间：2024-12-26 09:47:00【大中小】

LBIS® 小鼠卵清蛋白特异性免疫球蛋白E（OVA-IgE） ELISA试剂盒

LBIS® OVA-IgE Mouse

IgE（Immunoglobulin E，免疫球蛋白E）是第五个被发现的免疫球蛋白，由5个结构域（VH、CHε 1～4）构成的两个Hε链和两个L链中组成的IgE，其分子量约190000，电泳实验中向γ1域移动。IgE的代谢半衰期约3天，正常人血清中的IgE浓度非常低，约300 ng/mL。但在寄生虫感染和枯草热时浓度会升高。与过敏性相关的IgE被称作反应素。因接触过敏原导致反应素含量升高，反应素会在Fc域与存在于皮肤、呼吸道、消化脏器中嗜碱性粒细胞和肥大细胞的FcεR1受体结合，引起细胞过敏。在结合过敏原后细胞发生脱粒现象，组胺，五羟色胺，蛋白酶，肝素，趋化因子，前列腺素，白三烯等被投放，经过支气管收缩和黏膜水肿，分泌亢进，从而诱发支气管哮喘，部分荨麻疹，过敏性鼻炎，过敏性反应等I型过敏性反应。

本试剂盒是以OVA（卵清蛋白）作为免疫的抗原，在一个简化的反应系统中通过测定鼠抗OVA- IgE抗体值来进行小鼠免疫系统检测的试剂盒。

◆特点

●　测定时间短（总反应时间：1小时50分钟）。

●　微量样本即可测定。

●　使用无害的防腐剂。

●　全部试剂为溶液即用类型。

●　高测定精度和高重复性。

●　操作简便，无需进行特殊前处理。

试剂盒组成

组成品	状态	包装
OVA包被96孔板（干燥板）	清洗后使用	96 wells（8×12）／1个
标准溶液（Anti OVA-IgE:1, 200 U/mL）（单抗）	稀释后使用	100 μL/1瓶
缓冲液	直接使用	60 mL/1瓶
生物素结合抗小鼠IgE抗体（单抗）	稀释后使用	200 μL/1瓶
过氧化物酶·抗生素结合物	稀释后使用	200 μL/1瓶
显色液（TMB）	直接使用	12 mL/1瓶
终止液（1M H₂SO₄）※小心轻放	直接使用	12 mL/1瓶
浓缩清洗液（10×）	稀释后使用	100 mL/1瓶
孔板密封膜		3个
产品说明书		1本

◆样本信息

●　小鼠血清·血浆

●　10 μL/well（稀释样本）

※　样本需要用试剂盒附带的缓冲液调至标准曲线范围内。

※　样本必须稀释10倍以上。

◆测定范围

1.88～120 U/mL（标准曲线范围）

（本试剂盒中1 U/mL定义为抗原结合常数（Ka）为2.0×10⁸ M-1的抗体1.3 ng/mL）

◆实验数据

精度测试（组内变异）

样本	A	B
1	70.7	19.1
2	71.0	18.7
3	77.1	19.6
4	74.3	19.7
5	72.9	18.9
Mean	73.2	19.2
SD	2.6	0.42
CV（%）	3.6	2.2

单位：U/mL

重复性测试（组间变异）

测定日/样本	C	D	E
0天	60.0	15.0	3.75
1天	59.1	15.0	3.75
2天	58.1	14.7	3.65
3天	63.6	16.0	3.48
Mean	60.2	15.2	3.66
SD	2.4	0.57	0.13
CV（%）	4.0	3.7	3.4

单位：U/mL

加标回收测试

样本F

添加量	实测值	回收量	回收率（%）
0.00	6.93	—	—
5.35	12.2	5.27	98.5
10.7	17.8	10.9	102
17.1	25.1	18.2	106

单位：U/mL，n=3

样本G

添加量	实测值	回收量	回收率（%）
0.00	40.5	—	—
30.8	70.4	29.5	95.8
35.9	77.1	36.6	102
53.9	94.9	54.4	101

单位：U/mL，n=3

稀释直线性测试

2个血清样本连续用稀释缓冲液稀释3个梯度测定结果，直线回归值R²=0.9987~0.9999

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产品列表

产品编号	产品名称	产品规格
639-07651	（AKRIE-030）小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE) ELISA试剂盒 LBIS^® OVA-IgE Mouse	96 tests