Phos-tag(TM) SDS-PAGE相关产品

来源：作者：人气：-发表时间：2025-04-24 10:51:00【大中小】

Phos-tag™ SDS-PAGE相关产品

◆Reagents for Phos-tag™ SDS-PAGE gel preparation

Product Name	Pkg. Size	Wako Cat. No.	Use Application
30 w/v% Acrylamide/Bis Mixed solution(29:1)	500 mL	015-25635	Ready-to-use"Solution A".30%T, 3.3%C
Agarose H（High-strength type）	1 g	315-01203	High-strength Agarose has high strength even in a low-agarose environment and is suitable for electrophoretic migration of large nucleic acid fragments. It can be used in a concentration range of 0.2 - 1% and a separation range of 1 – 200 kbp.
	10 g	319-01201
	25 g	317-01202
10% SDS Solution	100 mL	311-90271	Ready-to-Use "Solution D"
10% SDS Solution	500 mL	313-90275	Ready-to-Use "Solution D"
Manganese(Ⅱ) Chloride Tetrahydrate, 99.0+ %（Titration）	25 g	134-15302	for Molecular Biology Please use for preparation of "Solution F"
Manganese(Ⅱ) Chloride Tetrahydrate, 99.0+ %（Titration）	100 g	136-15301
Zinc Chloride	25 g	268-01902	for Molecular Biology. Please use for preparation of "Solution M"
Bis-Tris	100 g	345-04741	Please use for preparation of "Solution N"
Sodium Hydrogensulfite〈JIS Special Grade〉	25 g	196-01372	Please use for preparation of "Solution O"
Sodium Hydrogensulfite〈JIS Special Grade〉	100 g	198-01371
Sodium Hydrogensulfite 〈For Molecular Biology〉	100 g	190-16461
MOPS	100 g	345-01804	Please use for preparation of "Solution P"
MOPS	250 g	341-01801
MOPS〈For Molecular Biology〉	100 g	341-08241
MOPS〈For Molecular Biology〉	500 g	343-08245
Separating Gel Buffer Solution (x4)	250 mL	192-11041	Ready-to-Use mixed solution of“Sol. B”and“Sol. D”for preparation of Resolving Gel. Contains SDS.
Stacking Gel Buffer Solution (x4)	250 mL	199-11051	Ready-to-Use mixed solution of“Sol.C”and“Sol. D”for preparation of Stacking Gel. Contains SDS.
N,N,N',N'-Tetramethylethylenediamine (TEMED)	25 mL	205-06313	for Electrophoresis
10 w/v% Ammonium Peroxodisulfate Solution (Ammonium Persulfate Solution)	25 mL	019-15922	Ready-to-Use“Solution G”

◆Premixed Buffers

Product Name	Pkg. Size	Wako Cat. No.	Use Application
Running Buffer Solution (x10)	1 L	184-01291	Ready-to-Use concentrated "Solution H"
SDS-PAGE 10x Running Buffer	1 L	312-90321	Ready-to-Use concentrated "Solution H"
SDS-PAGE 10x Running Buffer	5 L	318-90323	Ready-to-Use concentrated "Solution H"
SDS-PAGE Buffer, pH 8.5	5 L	192-16801	Ready-to-Use "Solution H", 1×buffer.
Tricine Running Buffer Solution (×10)	1 L	200-17071	Composition: 0.5 M Tris/0.5 M Tricine/1% SDS
Sample Buffer Solution (2ME+) (×2)	25 mL	196-11022	Sample buffer for Laemmli SDS-PAGE containing 2-mercaptoethanol
Sample Buffer Solution with 3-Mercapto-1,2-propanediol (×2)	25 mL	196-16142	Laemmli Sample Buffer containing 3-mercapto-1,2-propanediol (non-hazardous chemical) as substitute for 2-ME
AquaBlot™ 10×High Efficiency Transfer Buffer	1 L	015-26213	Can use it to transfer to a menbrane with high efficience.

◆Enzyme for Dephosphorylation

Product Name	Pkg. Size	Wako Cat. No.	Use Application
Alkaline Phosphatase（for Biochemistry）	50 U	018-10693	Applicable to dephosphorylation of proteins
Alkaline Phosphatase（for Biochemistry）	100 U	012-10691	Applicable to dephosphorylation of proteins

◆Reagents for Staining

Product Name	Pkg. Size	Wako Cat. No.	Use Application
Quick CBB Plus	250 mL	174-00553	Ready-to-Use Sol. K. Fixing and destaining procedure are not required. No organic solvents are necessary. Protein bands are stained in 10 minutes.
Quick CBB Plus	1 L	178-00551
Quick-CBB ?Staining solution A: 1L x 1 ?Staining solution B: 1L x 1	2 L	299-50101	By mixing staing solution A and B, ready-to-Use Sol. K
Silver Stain MS Kit	20 tests	299-58901	Modified proteins by glycosilation and/or phosphorylation can be detected sub-nanogram level on the electrophoretic gel.
Silver Stain Kit Wako	for 10 gels	299-13841	50~100 times more sensitive than CBB method.
Silver Stain Ⅱ Kit Wako	for 10 gels	291-50301	This kit contains Stopper, which can be adjusted the staining strength.
Negative Gel Stain MS Kit	20 tests	293-57701	Applicable to mass analysis and Western blot

◆Protein Size Marker

Product Name	Pkg. Size	Wako Cat. No.	Use Application
WIDE-VIEW^™ Prestained Protein Size Marker Ⅲ	25 μL	236-02463	A recommendable prestained marker used with Phos-tag™ SDS-PAGE because obtained bands are less distorted.
	500 μL	230-02461
	500 μL x3	234-02464

◆Positive Control（for confirmation of separation capacity of Phos-tag™ SDS-PAGE）

Product Name	Pkg. Size	Wako Cat. No.	Use Application
α-Casein, from BovineMilk, Dephosphorylated	1 mg	038-23221	Mixture of phosphylated and dephosphorylated α-casein
α-Casein, from BovineMilk, Dephosphorylated	10 mg	034-23223	Mixture of phosphylated and dephosphorylated α-casein

◆Electrophoresis Apparatus ? Precast Gels

Product Name	Pkg. Size	Wako Cat. No.	Use Application
EasySeparator	1 unit	058-07681	An electrophoresis tank for SuperSep precast polyacrylamide gels.
SuperSep Ace, 6%, 13 wells	10 gels	195-15171	Prior to use of SuperSep Phos-tag™ PAGE, please use these as sample confirmation. Expired in 9 months after manufacture
SuperSep Ace, 7.5%, 13 wells	10 gels	198-14941
SuperSep Ace, 7.5%, 17 wells	10 gels	191-14931
SuperSep Ace, 10%, 13 wells	10 gels	195-14951
SuperSep Ace, 10%, 17 wells	10 gels	192-14961
SuperSep Ace, 12.5%, 13 wells	10 gels	199-14971
SuperSep Ace, 12.5%, 17 wells	10 gels	196-14981
SuperSep Ace, 15%, 13 wells	10 gels	193-14991
SuperSep Ace, 15%, 17 wells	10 gels	190-15001

◆Reagents for Western Blotting

Product Name	Pkg. Size	Wako Cat. No.	Use Application
ImmunoStar LD＊ ?Luminescence solution A ?Luminescence solution B	200 cm²	296-69901	Highly sensitive (femto gram level) immunoblotting, utilizing detection by enhanced chemiluminescence using a unique luminol derivative L-012 as a substrate. Not available for sale in the US and Europe.
	1,000 cm²	292-69903
	2,000 cm²	290-69904
ImmunoStar Zeta＊	200 cm²	291-72401	Use for detection of proteins between the middle and low femto gram levels. Has stable, long-lasting luminescence signal.
	1,000 cm²	297-72403
	2,000 cm²	295-72404
ImmunoStar Basic＊	200 cm²	295-75101	A cost-effective, stable and long-lasting product.
	2,000 cm²	291-75103
	5,000 cm²	299-75104
Immuno Enhancer	2 assays	294-68601	Ready-to-Use Immunoreaction Enhancer for Western blotting and ELISA
	10 assays	290-68603
	40 assays	298-68604