订购货号 | 产品名称及规格 |
13020 | Cyanine5 NHS ester, 1 mg |
23020 | Cyanine5 NHS ester, 5 mg |
43020 | Cyanine5 NHS ester, 25 mg |
53020 | Cyanine5 NHS ester, 50 mg |
63020 | Cyanine5 NHS ester, 100 mg |
在过去的几年里,CY5荧光团已经成为生命科学研究和诊断领域流行的分子标记物。这些荧光素的较强发射光为红光,在这个范围大多数CCD检测器具有检测MAX灵敏度,而且是生物物质低背景区。染料的颜色是很强烈的,因此在凝胶电泳中低至1nmol的量都能被肉眼观测到。CY5 NHS ester是用于标记多肽、蛋白和寡核苷酸的氨基基团的活性染料。在标记反应中该染料需要少量的有机共溶剂(如DMF,DMSO)(请参照我们的推荐手册以获得更多帮助信息)。对于可溶性蛋白、各种各样的多肽和寡核苷酸而言,这种染料是较理想而且成本低廉的。该染料在有机溶剂对小分子物质的标记也是有效的。对于更多的精细目标物,如易降解蛋白,如果DMF或DMSO会对其有影响,可以考虑water-soluble Cy5 NHS ester(水溶性Cy5 NHS ester染料),因为它们不需要有机溶剂助溶,而且具有和荧光染料类似的性质。
CY5荧光团可以与很多的仪器如荧光显微镜、成像仪、扫描仪、荧光分析仪等相兼容。许多的CY5衍生物——CY5 NHS ester可以替代Alexa Fluor 647和 DyLight 649等活化酯(activated esters)。
S" style="color: rgb(61, 61, 61);">General properties
Appearance: | dark blue powder |
Molecular weight: | 616.19 |
Molecular formula: |
C36H42ClN3O4 |
CAS number: | 1032678-42-4, 350686-88-3 |
Solubility: | good in polar (DMSO, DMF) and chlorinated (DCM, chlroform) organic solvents, low solubility in water |
Quality control: | NMR 1H (95%) and 13C, TLC, functional testing |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Excitation maximum, nm: | 646 |
Extinction coefficient at excitation maximum, Lmol-1cm-1: | 250000 |
Emission maximum, nm: | 662 |
Fluorescence quantum yield: | 0.2 |
Product citations
- Wilkinson, N.; Metaxas, A.; Ruud, E.; Raethke, E.; Wickramaratne, S.; Reineke, T.M.; Dutcher, C.S. Internal structure visualization of polymer – clay flocculants using fluorescence. Colloids and Interface Science Communications, 2016, 10–11, 1–5. doi:10.1016/j.colcom.2016.03.002
- Chen, K.; Chen, Q.; Wang, K.; Zhu, J.; Li, W.; Li, W.; Qiu, L.; Guan, G.; Qiao, M.; Zhao, X.; Hu, H.; Chen, D. Synthesis and characterization of a PAMAM-OH derivative containing an acid-labile β-thiopropionate bond for gene delivery. International Journal of Pharmaceutics, 2016, 509(1–2), 314–327. doi: 10.1016/j.ijpharm.2016.05.060
- Wu, F.; Liu, C.; Chen, Y.; Yang, S.; Xu, J.; Huang, R.; Wang, X.; Li, M.; Liu, W.; Mao, W.; Zhou, X. Visualization of G-quadruplexes in gel and in live cells by a near-infrared fluorescent probe. Sensors and Actuators B: Chemical, 2016, 236, 268&?β?β牥戭硯;????鑈αndash;275. doi:10.1016/j.snb.2016.05.162
- Ray, J.; Shin, I.; Ilgu, M.; Bendickson, L.; Gupta, V.; Kraus, G.A.; Nilsen-Hamilton, M. IMAGEtags: Quantifying mRNA Transcription in Real Time with Multiaptamer Reporters. Methods in Enzymology, 2016, 572, 193–213. doi:10.1016/bs.mie.2016.02.028
- Hartley, J.M.; Zhang, R.; Gudheti, M.; Yang, J.; Kope?ek, J. Tracking and quantifying polymer therapeutic distribution on a cellular level using 3D dSTORM. Journal of Controlled Release, 2016, 231, 50–59. doi: 10.1016/j.jconrel.2016.02.005
- Xu, X.; Li, L.; Zhou, Z.; Sun, W.; Huang, Y. Dual-pH responsive micelle platform for co-delivery of axitinib and doxorubicin.International Journal of Pharmaceutics, 2016, 507(1–2), 50–60. doi: 10.1016/j.ijpharm.2016.04.060
- Tran, T.-H.; Krishnan, S.; Amiji, M.M. MicroRNA-223 Induced Repolarization of Peritoneal Macrophages Using CD44 Targeting Hyaluronic Acid Nanoparticles for Anti-Inflammatory Effects. PLoS One, 2016, 11(5), e0152024. doi: 10.1371/journal.pone.0152024
- Liu, C.; Stonestrom, A.J.; Christian, T.; Yong, J.; Takase, R.; Hou, Y.-M.; Yang, X. Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction. Journal of Biological Chemistry, 2016, 291(19), 10426–10436. doi: 10.1074/jbc.M115.697789
- Li, L.; Sun, W.; Zhang, Z.; Huang, Y. Time-staggered delivery of docetaxel and H1S6A,F8A peptide for sequential dual-strike chemotherapy through tumor priming and nuclear targeting. Journal of Controlled Release, 2016, 232, 62–74. doi:10.1016/j.jconrel.2016.04.021
- Leenders, J.; Baker, M.B.; Pijpers, I.; Lafleur, R.; Albertazzi, L.; Palmans, A.R.A.; Meijer, E.W. Supramolecular polymerisation in water; elucidating the role of hydrophobic and hydrogen-bond interactions. Soft Matter, 2016, 12, 2887–2893. doi:10.1039/c5sm02843d
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